Histocytology and Plant Cell Imaging platform
The Histocytology and Cellular Imaging Platform (PHIV) is located on two sites, distant of 3 km : PHIV-La Gaillarde located on the INRAE-Supagro Campus (map) and PHIV-Lavalette located on the CIRAD campus (map).
PHIV (http://phiv.cirad.fr/) is a shared platform between the two research institut IPSiM and AGAP (http://umr-agap.cirad.fr/en). Since 2003, it belongs to the regional platform «Montpellier Ressources Imagerie» (http://www.mri.cnrs.fr/) federating plant and animal imaging platforms at Montpellier. PHIV was quality certified in 2008 by the national committee for common services (CNOC) from INRAE, and this certification was renewed in 2013.
In the acknowledgments and / or in the Materials and Methods section, the technical contribution of the PHIV platform must be underlined and clearly mentioned and the personnel directly involved, when they are not co-authors, can be named.
Example for PHIV: “We acknowledge the Histocytology and Plant Cell Imaging platform (PHIV) for technical support.”
Example for MRI: “We acknowledge the imaging facility MRI, member of the national infrastructure France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04, «Investments for the future»)“
[ Acknowledging core facilities ]
Access
Please contact :
Carine Alcon
(+33) 499612608, carine.alcon@supagro.fr
Contact: umr-ipsim-phiv@supagro.fr
To use the advanced core facilities (confocal and multiphotonic microscopy, high content screening, RX tomography, electron microscopy and cytometry) you have to subscribe to the MRI plateform http://www.mri.cnrs.fr/
Assisting users in providing:
Protocols, technical assistance, expertise
Experiment feasibility, planification, and access to equipments and ressources
Experiment quotation
Staff
| La Gaillarde | ||||
|---|---|---|---|---|
| scientific manager | Alexandre MARTINIERE | 0499612021 | UMR BPMP | alexandre.martiniere-delaunay@supagro.fr |
| team | Carine ALCON | 0499612608 | UMR BPMP | carine.alcon@supagro.fr |
| team | Tou Cheu XIONG | 0499613042 | UMR BPMP | toucheu.xiong@inra.fr |
| Lavalette | ||||
| scientific manager | Jean-Luc VERDEIL | UMR AGAP | verdeil@cirad.fr | |
| team | Matthieu DEJEAN | UMR AGAP | matthieu.dejean@cirad.fr | |
| team | Christelle BAPTISTE | UMR AGAP | christelle.baptiste@cirad.fr | |
| team | Frédéric GATINEAU | UMR AGAP | frederic.gatineau@cirad.fr | |
| team | Sergi NAVARRO SANZ | UMR AGAP | sergi.navarro_sanz@cirad.fr | |
| secretarial office | Valérie CARUANA | UMR AGAP | valerie.caruana@cirad.fr |
Expertise
Specimen preparations : inclusions, sectioning, and stainings
Molecular histology /In situ hybridization / immunofluorescence)
In vivo imaging, use of fluorescent biosensors
Optical microscopy: wide field, confocal, multiphoton
spectrofluorometry
Image analysis
Equipment / Technologies
| La Gaillarde | Lavalette |
|---|---|
| microscope confocal leica sp8 microscope zeiss observer 7 apotome microscope zeiss axiovert 200m macroscope axiozoom V16 microscope olympus bx61 camera hamamastu bioluminescence microscope olympus bh2 stereomicroscope olympus szx16 microtome RM2165 stereomicroscope zeiss stemi 2000c knifemaker lkb Vibratome Biorad H1200 |
automate zeiss 880 cryostat hm 525 droit leica dm4500 2 droit nikon eclipse NI-E 2 histoprocesseur histos 5 centre dinclusion EC350 insitupro vsi microdissecteur leica lmd7000 microtome leica rm2245 vibratome hm650v |
training
- Transport membranaire chez les plantes
- MISTRAL : Montpellier International School on ion and water TRansport in plants
- Biocampus
News
Significants publications
Robe K, Stassen M, Watanabe S, Espadas J, Gonzalez P, Rossille A, Li M, Hem S, Roux A, Santoni V, Chamieh J, Dubos C✉, Izquierdo E✉ (2025) Coumarin-facilitated iron transport: an IRT1 independent strategy for iron acquisition in Arabidopsis thaliana. Plant Commun.
, (in press)
Lecuyer C*, Vettor A*, Fizames C, Javot H, Martin A, Mazouzi M, Cortijo S✉ (2025) Establishment, maintenance and consequences of inter-individual transcriptional variability for a gene involved in nitrate nutrition in plants. bioRxiv,
Gorgues L, Smokvarska M, Mercier C, Igisch CP, Crabos A, Dongois A, Bayle V, Fiche J-B, Nacry P, Nollmann M, Jaillais Y, Martinière A✉ (2025) GEF14 acts as a specific activator of the plant osmotic signaling pathway by controlling ROP6 nanodomain formation. EMBO Rep., 26(8):2146-2165
Danek M*, Hdedeh O*, Boutet J, Safi H, Abuzeineh A, Martín-Barranco A, Fiche J-B, Mercier C, Nollmann M, Boutté Y, Santoni V, Mongrand S, Martinière A, Zelazny E✉ (2025) Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2. bioRxiv,
Lee Y*, Demes-Causse E*, Yoo J, Jang SY, Jung S, Jaślan J, Hwang G-S, Yoo J, De Angeli A, Lee S✉ (2025) Structural basis for malate-driven, pore lipid-regulated activation of the Arabidopsis vacuolar anion channel ALMT9. Nat. Commun., 16:1817
Alcon C, Comte A, Curie C, Xiong TC✉ (2024) Imaging of labile Fe2+ and Fe3+ in living Arabidopsis thaliana roots. Plant Physiol., 195(4):2520-2523
Gómez-Gallego T, Molina-Luzón MJ, Conéjéro G, Berthomieu P, Ferrol N✉ (2024) The arbuscular mycorrhizal fungus Rhizophagus irregularis uses the copper exporting ATPase RiCRD1 as a major strategy for copper detoxification. Environ. Pollut., 341:122990
Robe K, Conéjéro G, Dubos C✉ (2023) The use of spectral imaging to follow the iron and pH-dependent accumulation of fluorescent coumarins. Methods Mol. Biol., 2665:23-30
Smokvarska M, Bayle V, Maneta-Peyret L, Fouillen L, Poitout A, Dongois A, Fiche J-B, Gronnier J, Garcia J, Höfte H, Nolmann M, Zipfel C, Maurel C, Moreau P, Jaillais Y, Martinière A✉ (2023) The receptor kinase FERONIA regulates phosphatidylserine localization at the cell surface to modulate ROP signaling. Sci. Adv., 9(14):eadd4791
Alcon C, Xiong TC (2023) Calcium live imaging at multi-scales from cellular to organ level in Arabidopsis thaliana. in Ivan Couée (Eds): Plant Abiotic Stress Signaling. Methods Mol. Biol., 2642:85-95
Grant-Grant S, Schaffhauser M, Baeza-González PA, Gao F, Conéjéro G, Vidal EA, Gaymard F, Dubos C, Curie C, Roschzttardtz H✉ (2022) B3 transcription factors determine iron distribution and FERRITIN gene expression in embryo but do not control total seed iron content. Front. Plant Sci., 13:870078
Then C*, Bellegarde F*, Schivre G*, Martinière A, Macia J-L, Xiong TC✉, Drucker M✉ (2021) Plant viruses can alter aphid-triggered calcium elevations in infected leaves. Cells, 10(12):3534
Castaings L
, Alcon C, Kosuth T, Correia D, Curie C (2021) Manganese triggers phosphorylation-mediated endocytosis of the Arabidopsis metal transporter NRAMP1. Plant J., 106(5):1328-1337
Bayle V*, Fiche J-B*, Burny C, Platre MP, Nollmann M, Martinière A, Jaillais Y (2021) Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues. Nat. Protoc., 16(3):1600-1628
Robe K, Conéjéro G, Gao F, Lefebvre-Legendre L, Sylvestre-Gonon E, Rofidal V, Hem S, Rouhier N, Barberon M, Hecker A, Gaymard F, Izquierdo E, Dubos C (2021) Coumarin accumulation and trafficking in Arabidopsis thaliana: a complex and dynamic process. New Phytol., 229(4):2062-2079
Maghiaoui A*, Bouguyon E*, Cuesta C, Perrine-Walker F, Alcon C, Krouk G, Benková E, Nacry P, Gojon A, Bach L (2020) The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. J. Exp. Bot., 71(15):4480-4494
Smokvarska M*, Charbel F*, Platre MP*, Fiche J-B, Alcon C, Dumont X, Nacry P, Bayle V, Nollmann M, Maurel C, Jaillais Y, Martinière A✉ (2020) A plasma membrane nanodomain ensures signal specificity during osmotic signaling in plants. Curr. Biol., 30(23):4654-4664.e4
Martinière A*, Fiche J-B*, Smokvarska M, Mari S, Alcon C, Dumont X, Hematy K, Jaillais Y, Nollmann M, Maurel C (2019) Osmotic stress activates two reactive oxygen species pathways with distinct effects on protein nanodomains and diffusion. Plant Physiol., 179(4):1581-1593
Martinière A, Bassil E, Jublanc E, Alcon C, Reguera M, Sentenac H, Blumwald E, Paris N (2013) In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system. Plant Cell, 25(10):4028-4043
Roschzttardtz H, Conéjéro G, Divol F, Alcon C, Verdeil J-L, Curie C, Mari S (2013) New insights into Fe localization in plant tissues. Front. Plant Sci., 4:350
Quality
Montpellier Ressources Imagerie is a multi-site Technological Platform of UMS Biocampus, certified ISO 9001-NFX 50-900
