Equipment:

microscope confocal Leica SP8

Charge:

Tariff (public) : 34.36 €/hour

Tariff (private) : 98.46 €/hour

Associated technologies:

3D imaging

FRAP photoactivation

Spectral imaging

Brief description:

motorized inverted confocal microscope

multidimensional scan mode

3D imaging on fixed or live cell sample

suitable for F-techniques (FRAP – FRET)
 

Technical description:

 

objectives

10X HC Plan Fluotar 0.3 NA
20X HC Plan Apochromat 0.7 NA CS
40X HCX corr Plan Apochromat CS 1.1 NA water
40X HCX Plan Apochromat CS 1.3 NA oil
63X HCX Plan Apochromat CS 1.4 NA oil

 

exciation source : Lamp HXP120
Lasers : 405, Argon (458, 488, 514), 561, 633
Filters cubes
DAPI (Ex BP340-380, Em LP425
FITC (Ex Bp450-490, Em LP515
GFP (Ex BP480/40, Em BP527/30)
DsRed Et,k (Ex BP546/12, Em BP605/75)
spectral acquisition in confocal mode
Détectors : 1 PMT and 2 PMTs hybride HyD GaAsP multi-dimensional scan mode: XYZ, XZ etc… lambda, time lapse, mark and find, tile scan
FRAP / FRET