Histocytology and Plant Cell Imaging platform

The Histocytology and Cellular Imaging Platform (PHIV) is located on two sites, distant of 3 km : PHIV-La Gaillarde located on the INRAE-Supagro Campus (map) and PHIV-Lavalette located on the  CIRAD campus (map).

PHIV (http://phiv.cirad.fr/) is a shared platform between the two research institut IPSiM and AGAP (http://umr-agap.cirad.fr/en). Since 2003, it belongs to the regional platform «Montpellier Ressources Imagerie» (http://www.mri.cnrs.fr/) federating plant and animal imaging platforms  at Montpellier. PHIV was quality certified in 2008 by the national committee for common services (CNOC) from INRAE, and this certification was renewed in 2013.

In the acknowledgments and / or in the Materials and Methods section, the technical contribution of the PHIV platform must be underlined and clearly mentioned and the personnel directly involved, when they are not co-authors, can be named.
Example for PHIV: “We acknowledge the Histocytology and Plant Cell Imaging platform (PHIV) for technical support.”
Example for MRI: “We acknowledge the imaging facility MRI, member of the national infrastructure France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04, «Investments for the future»)
[ Acknowledging core facilities ]


Please contact :
Carine Alcon
(+33) 499612608, carine.alcon@supagro.fr
Contact: umr-ipsim-phiv@supagro.fr

To use the advanced core facilities (confocal and multiphotonic microscopy, high content screening, RX tomography, electron microscopy and cytometry) you have to subscribe to the MRI plateform http://www.mri.cnrs.fr/


Assisting users in providing:

Protocols, technical assistance, expertise

Experiment feasibility, planification, and access to equipments and ressources

Experiment quotation

La Gaillarde
scientific manager Alexandre MARTINIERE 0499612021 UMR BPMP alexandre.martiniere-delaunay@supagro.fr
team Carine ALCON 0499612608 UMR BPMP carine.alcon@supagro.fr
team Tou Cheu XIONG 0499613042 UMR BPMP toucheu.xiong@inra.fr
scientific manager Jean-Luc VERDEIL   UMR AGAP verdeil@cirad.fr
team Matthieu DEJEAN   UMR AGAP matthieu.dejean@cirad.fr
team Christelle BAPTISTE   UMR AGAP christelle.baptiste@cirad.fr
team Frédéric GATINEAU   UMR AGAP frederic.gatineau@cirad.fr
team Sergi NAVARRO SANZ   UMR AGAP sergi.navarro_sanz@cirad.fr
secretarial office Valérie CARUANA   UMR AGAP valerie.caruana@cirad.fr


Anatomy and histology
Specimen preparations : inclusions, sectioning, and stainings
Molecular histology /In situ hybridization / immunofluorescence)
In vivo imaging, use of fluorescent biosensors
Optical microscopy: wide field, confocal, multiphoton
Image analysis
Thematic schools


Significants publications

Then C*, Bellegarde F*, Schivre G*, Martinière A, Macia J-L, Xiong TC, Drucker M (2021) Plant viruses can alter aphid-triggered calcium elevations in infected leaves. Cells, 10(12):3534

Castaings L✉, Alcon C, Kosuth T, Correia D, Curie C✉ (2021) Manganese triggers phosphorylation-mediated endocytosis of the Arabidopsis metal transporter NRAMP1. Plant J., 106(5):1328-1337

Bayle V*, Fiche J-B*, Burny C, Platre MP, Nollmann M, Martinière A✉, Jaillais Y✉ (2021) Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues. Nat. Protoc., 16(3):1600-1628

Robe K, Conéjéro G, Gao F, Lefebvre-Legendre L, Sylvestre-Gonon E, Rofidal V, Hem S, Rouhier N, Barberon M, Hecker A, Gaymard F, Izquierdo E✉, Dubos C✉ (2021) Coumarin accumulation and trafficking in Arabidopsis thaliana: a complex and dynamic process. New Phytol., 229(4):2062-2079

Maghiaoui A*, Bouguyon E*, Cuesta C, Perrine-Walker F, Alcon C, Krouk G, Benková E, Nacry P, Gojon A, Bach L✉ (2020) The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. J. Exp. Bot., 71(15):4480-4494

Smokvarska M*, Charbel F*, Platre MP*, Fiche J-B, Alcon C, Dumont X, Nacry P, Bayle V, Nollmann M, Maurel C, Jaillais Y, Martinière A (2020) A plasma membrane nanodomain ensures signal specificity during osmotic signaling in plants. Curr. Biol., 30(23):4654-4664.e4

Martinière A*✉, Fiche J-B*, Smokvarska M, Mari S, Alcon C, Dumont X, Hematy K, Jaillais Y, Nollmann M, Maurel C (2019) Osmotic stress activates two reactive oxygen species pathways with distinct effects on protein nanodomains and diffusion. Plant Physiol., 179(4):1581-1593

Martinière A, Bassil E, Jublanc E, Alcon C, Reguera M, Sentenac H, Blumwald E, Paris N✉ (2013) In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system. Plant Cell, 25(10):4028-4043

Roschzttardtz H, Conéjéro G, Divol F, Alcon C, Verdeil J-L, Curie C, Mari S✉ (2013) New insights into Fe localization in plant tissues. Front. Plant Sci., 4:350




Montpellier Ressources Imagerie is a multi-site Technological Platform of UMS Biocampus, certified ISO 9001-NFX 50-900

PHIV-MRI webinar: Julio Mateos-Langerak

Apr 27, 2021 – Managing micrsocopy data “fairly” with OMERO

Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues

Super-resolution microscopy techniques have pushed the limit of optical imaging to unprecedented spatial resolutions. However, one of the frontiers in nanoscopy is its application to intact living organisms. Here we describe the implementation and application of...


July 2-13, 2018 – Montpellier International School on ion and water TRAnsport in PLant

Seminar microscopy – Sylvain Derossi

Jun 18, 2018 – SPIM (light sheet microscopy) – MRI platform