Equipment:

microscope Zeiss observer 7 / ApoTome

Associated technologies:

Wide field fluorescence microscopy

DIC transmitted light imaging for the  objectives 40X et 60X

Brief description:

Inverted microscope equiped with a motorized stage and a structurated illumation device to improve the axial resolution in fluorescence imaging

Z stack, tiles, timelapse

Technical description:

 

Objectives :

5X      EC Plan-Neofluar   0,16NA

10x    EC Plan-Neofluar   0,3NA

20X    Plan-Apochromat                 0,8NA

40x     Plan-Apochromat   DIC      1,3 NA   oil

63X    Plan-Apochromat    DIC     1.4 NA   oil

Light source : Xcite 120 LED

 

Filters wheel :

position 1 : Ex BP 325 – 390 BS FT 395 Em BP 445/50

position 2 : Ex BP 436/20 BS FT 455 Em BP 480/40

position 3 : Ex BP 470/40 BS FT 495 Em BP 525/50

position 4 : Ex BP 545/25 BS FT 570 Em BP 605/70

position 5 : BP 538/40  BS FT 570 em BP 600/50

detector :

monochrome Camera Hammamastu Orca Fllash 4

software : Zen 2