Equipment:

microscope Zeiss Axiovert 200M

Associated technologies:

Wide field fluorescence microscopy, Vidéomicroscopy

dedicated for ratiometric fluorescent probes imaging

Brief description:

Inverted microscope with a monochrome camera coolsnapHQ

LED light source which allows a fast dual excitation and an external filter wheel to switch quickly between two emission wavelengths

 

Technical description:

 

Objectives :

20X A Plan Apochromat 0.3 NA

40X A Fluar 1.3 NA oil

 

Light source: LED lumencor spectra 7

Filter wheel :

position 1 : BS HC BS 458,

position 2 : BS HC BS 495 Em BP 525/ 45

position 3 : BS HC BS 506 Em BP 536 / 40

position 4 : BS T 565LPXR Em BP 600/50

position 5 : BS 434-447 / 510-520

external wheel Sutter lamda10B : position 1 BP 480/40

position2 BP 535 /30

Detector :

monochrome camera Photometrics CoolSnap HQ

 Software : metafluor