Droplet digital PCR (ddPCR) is a molecular biology technology enabling absolute and precise nucleic acid quantification. By partitioning samples into thousands of microdroplets, each acting as an independent PCR reaction, ddPCR allows direct digital quantification of DNA or RNA using statistics laws. This enhances sensitivity and robustness, making it ideal for detecting rare mutations, monitoring gene expression, analyzing genetic variations, and quantifying pathogens.

In this three-part seminar, Martial Saumier (Bio-Rad) will present the technology, its equipment, multiplexing strategies, and broader applications. Gael Petitjean (Benkirane team and head of digital PCR plateform at IGH Montpellier) will discuss ddPCR applications in gene expression analysis. Willy Aubert will focus on its use in mutant screening of a white lupin TILLING population targeting SNPs at IPSiM.

Martial Saumier (BioRad): Presentation of Droplet Digital PCR and the opportunities offered by this technology

Gael Petitjean (IGH Montpellier CNRS): Presentation of Digital PCR at IGH for gene expression monitoring and multiplexing cases

Aubert Willy (IPSiM Plasticité): Implementation and development of Digital PCR for the screening of new mutants of interest in a Lupinus albus TILLING population