IBIP seminar
The seminars take place on the Montpellier Institut Agro/INRAE Campus of La Gaillarde (2, place P. Viala Montpellier)
Monday, December 02, 2024 at 2:00 PM – Amphi 208 Cœur d’Ecole
Kevin Bellande
(Laboratory of Molecular and Cellular Biology – Switzerland)
Conserved developmental trajectories channeling lateral root primordium morphogenesis
Improving crop resilience through enhanced root system architecture (RSA) is critical for adaptation to changing environmental conditions. Lateral root (LR) formation, which is predominantly regulated by auxin signalling pathways, is a complex process. We recently presented the first developmental atlas of LR development in the model grass species Brachypodium distachyon (Bd), revealing the reactivation of the endodermal cell cycle that contributes to the formation of root cap and columella cells in emerging LRs. Interestingly, our results suggest that auxin signalling, as indicated by DR5 promoter activity, is not evident in the phloem pole pericycle or during early LR development, but correlates with cell wall modifications during the emergence of the LR primordium (LRP).
In a comparative analysis of Bd21 and Bd21-3 accessions, we observed similar LRP synchronisation after root tip removal, but distinct LR architecture thereafter. RNAseq analysis revealed distinct early and late responses in both accessions, with significant overlap of differentially expressed genes at later time points. An enrichment of cell wall related processes was observed and patterns of cell wall remodelling gene families varied during early LR formation. In addition, we identified several GDSL/GELP genes with similar expression patterns during LR formation in both Brachypodium and Arabidopsis, as well as conserved GDSL protein structures. Notably, the orthologous copy of AtGELP38, identified as a suberin synthase in Arabidopsis, BdGELP38, was expressed in the endodermis overlying the LRPs and functionally rescued the absence of the suberin phenotype in gelpquint mutants.
Dissecting the trajectories of cell types and whether there are regulatory mechanisms that could be part of conserved modules for root branching in general would be of further interest.
Bellande Kevin1,2 * ; De Jesus Vieira Teixeira Cristovāo1 ; Marc-Martin Sophie1 ; Badet Thomas3 ; Péret Benjamin2 ; E.M. Vermeer Joop1 *
1 Laboratory of Molecular and Cellular Biology, Institute of Biology, University of Neuchâtel, Rue Emile Argand 11, CH-2000, Neuchâtel, Switzerland.
2 IPSiM, University of Montpellier, CNRS, INRAE, Institut Agro, Montpellier, France.
3 Laboratory of Evolutionary Genetics, Institute of Biology, University of Neuchâtel, Rue Emile Argand 11, CH-2000, Neuchâtel, Switzerland.
* Correspondances : kevin.bellande@cnrs.fr; josephus.vermeer@unine.ch
Contact : benjamin.peret@cnrs.fr