n the Mesoscopic Imaging Facility (MIF) at EMBL Barcelona, researchers study the details of biological systems in the context of organs, body parts, or entire organisms.
Light-sheet microscopy produces images of biological samples one thin slice at a time. This minimises photodamage caused by the excitation radiation used to illuminate the sample. The sample emits fluorescence, which is collected for each slice by the detection optics. At the end of this process, the light-sheet images obtained along the entire depth of the sample are stacked on top of one another. You can visualise them as if you were leafing through a flip book.
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