Séminaire INTERNE
Jeudi 14 novembre – Salle Bibliothèque IBIP à 14h00

Françoise GOSTI
B&PMP-Equipe Métaux

Plant Defensin Type 1 (PDF1) expression and plant response to biotic and abiotic stresses.

The Metal phytotoxicity team study plant adaptation to metals taking advantage of extremophile species, which can tolerate and hyperaccumulate metals without sign of toxic symptoms. We use molecular genetic approaches to decipher gene expression modification and genomic organisation occurring in A. halleri extremophile as compared to the closely related model A. thaliana. In parallel, we perform biochemical analysis to understand protein mode of action.

This lead to the discovery that, beside their role in plant response to pathogens, plant defensins are part of the arsenal developed by plants to withstand metal toxicity. Ectopic expression of defensins correlates with plant increased capacity to withstand both abiotic and biotic stresses and defensin expression is also constitutively higher in extremophille species. However, studies are still rare to explore if endogenous defensin expression decrease would affect these different properties in the same way and/or if similar or different signalling pathways are involved.

We thus undertook a genetic artificial micro RNA (amiRNA) approach to decrease expression of several members of the A. thaliana Plant Defensin type 1 family (AtPDF1) and performed physiological and biochemical comparative characterisation of wild-type (WT) and amiRNA transgenic lines. We will present our current results and hypothesis on unexpected amiRNA transgenics response to biotic (B. cinerea pathogen attack) and abiotic (zinc excess) stresses.

 contact : francoise.gosti@supagro.fr

Séminaires à venir :
https://www1.montpellier.inra.fr/wp-inra/bpmp/actualites/ rubrique Séminaires (2015-220)
Jeudi 21 novembre séminaire interne : Fei Gao équipe FEROS
Jeudi 28 novembre : Akihiro Saïto (contact stephane.mari@inra.fr)

Jeudi 12 décembre : Ivan Baxter (contact: hatem.rouached@inra.fr)

Contacts IBIP :  Sabine Zimmermann (sabine.zimmermann@cnrs.fr), Alexandre Martiniere (alexandre.martiniere@cnrs.fr), Florent Pantin (florent.pantin@supagro.fr), Chantal Baracco (chantal.baracco@inra.fr)