Seminar IBIP – thématique « Association genetics in plants »
Les séminaires ont lieu sur le Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)
Mercredi 26 novembre 2014
Amphithéatre Philippe Lamour de 10h45 à 12h
Dissecting quantitative regulation of root growth using systems genetics
Gregor Mendel Institute, Vienna, Austria
One of the ultimate challenges of biology is to understand how genotypes translate into phenotypes. Recent technological leaps in DNA sequencing have brought tremendous progress in this area. However, compared to the progress on acquiring genome sequences, our comprehension of genotype to phenotype relations is growing very slowly, especially in complex multicellular organisms such as plants, in which specific biological processes often occur only temporarily and are restricted to specific organs, tissues or even individual cells. One bottleneck is the ability to accurately phenotype a large number of individuals and genotypes in a temporally and spatially resolved manner. Another major intricacy is to identify and comprehend dynamic networks of interacting genes. As a model to address these challenges, we use the root of Arabidopsis thaliana and try to dissect how root growth is tuned by the activities of genes and their networks. For this we have developed several high ‐ throughput methods to accurately quantify multiple traits describing root growth and development over periods of time and at different scales. With these pipelines, we assess the trait variation that is present in natural and artificial populations of Arabidopsis. Using genome ‐ wide association mapping combined with the analysis of expression and other large ‐ scale – omics data, we determine genes, their alleles and the networks that act to quantitatively regulate root developmental and growth processes. With these methods we have recently identified multiple novel regulators and networks that tune root growth.