Les séminaires ont lieu sur le Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)
Jeudi 7 novembre 2013
Amphi 206 (Cœur d’Ecole) à 14h
Dissecting quantitative regulation of root growth using large-scale phenotyping
Dr Wolfgang Busch
Gregor Mendel Institute, Vienne
One of the ultimate major bottleneck lies in the ability to phenotype a large number of individuals and genotypes with high accuracy. This is particularly lagging in complex multicellular organisms such as plants, in which specific biological processes often occur only temporarily and are restricted to specific organs, tissues or even individual cells. Further significant progress in unraveling the genetic bases of many relevant traits will require the ability to assess phenotypes in a temporally and spatially resolved manner and with a high throughput. Imaging based phenotyping methods are powerful tools to address these challenges. We have developed several methods to accurately quantify multiple traits describing root growth and development over periods of time and at different scales with a high-throughput. With these pipelines, we assess trait variation that is present in natural and artificial populations of Arabidopsis thaliana and can subsequently assess the phenotypic variation caused by sequence variation. Using natural variation and genome wide association mapping, we determined genome regions underlying the phenotypic variation at the cellular and organ scale level under multiple environmental conditions. By using independent expression data we could identify multiple novel regulators that quantitatively regulate developmental and growth processes.
Contact : Laurence Lejay