Les séminaires ont lieu sur le Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)
Jeudi 18 septembre 2014
Amphi 208 (Cœur d’Ecole) à 14h
New genetic tools to study bacterial wilt caused by Ralstonia solanacearum on potato
Universitat de Barcelona and Centre for Research in Agricultural Genomics (CRAG), Barcelona, Catalonia, Spain
Solanum commersonii is a wild potato species that exhibits significant resistance to bacterial wilt (or potato brown rot), a devastating disease caused by Ralstonia solanacearum. I will describe three complementary approaches in bacteriology and plant molecular biology to gain insight into the molecular dialogue between S. comersonii and R. solanacearum during infection. 1- I will present our new findings on the regulation of R. solanacearum virulence genes, obtained using a newly-developed gene reporter system 2- I will describe a simple, high-throughput method that we have developed for quantitative detection of bacterial colonisation in planta, using a light-emitting R. solanacearum reporter strain. This non-disruptive methodology also allows for the identification of latent infections on symptomless plants and can be applied to evaluate potato germplasm for resistance to R. solanacearum in breeding programmes. 3- I will show our recent data on the characterisation of the R. solanacearum type III secretion effector AWR5 through heterologous expression in yeast. We hypothesise that this bacterial protein is delivered into plant host cells to interfere with the central metabolic regulator Tor (Target of Rapamycin).
Contact : Laurence Marquès