Les séminaires ont lieu sur le Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)
Jeudi 14 octobre 2010
Amphi 208 (Cœur d’Ecole) à 14h
Bioimaging techniques used in the study of plant membrane protein dynamics
John Runions & Alexandre Martiniere
Senior Lecturer in Cell Biology, Oxford Brookes University
We use a variety of different approaches for studying membrane proteins. In this talk, wepresent the technology that enables us to study membrane protein associations anddynamics. The majority of these techniques employ fluorescent proteins for studyingprotein function in living cells. The plant cell secretory pathway compartments that westudy include endoplasmic reticulum, Golgi apparatus, and the plasma membrane. Ourmost commonly used approach is to bleach (FRAP) or photoactivate fluorescent proteinsthat are fused to the membrane proteins under study. Other techniques currently beingused in our lab include FRET/FLIM, TIRF, and laser tweezers. We will present results fromseveral recent studies. In particular, we will describe the discovery of a bridge formedbetween the plasma membrane and the cell wall by the cytoskeletal nucleating proteinFORMIN 1. Other research highlights include altered dynamics of the aquaporin PIP2;1under conditions of increased salt stress; the discovery that FAD2 acts as a temperaturesensor; and our finding that PIN2 localisation seems to be affected by the sterolcomposition of the plasma membrane. The confocal microscopy techniques that weemploy, therefore, lend themselves to many types of research including cytoskeletoninteractions in cells signalling, circadian dynamics of membrane composition, andhormone receptor function.
Contact : Doan Luu