Séminaire IBIP
Les séminaires ont lieu sur le Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)

Vendredi 23 mai 2008
Amphi 206 (Cœur d’Ecole) à 14h

Transcription regulation of early nodulin genes by Rhizobium Nod factors

Fernanda De Carvalho-Niebel
Laboratoire des Interactions Plantes-Microorganismes – INRA-CNRS Toulouse

Rhizobium lipo-chitooligosaccharides known as Nod factors (NF) play a key role in the establishment of the symbiotic interaction that leads to nodule development in legumes. NF signal perception and transduction involves multiple receptor-like kinases and oscillatory calcium changes that are necessary for the subsequent transcriptional activation of early nodulingenes. We have focused our studies on the molecular dissection of the regulatory mechanisms underlying host gene expression by symbiotic NFs, in root hairs of the model legume Medicago truncatula. Functional promoter studies of the well characterized NF-elicited MtENOD11 gene has led to the identification of the first “NF-box” responsive sequence sufficient to direct NF-mediated expression in root hairs. Following a yeast one-hybrid screen of root hair-specific cDNA libraries, three NF-box-interacting factors belonging to a so far uncharacterized group of the AP2/ERF transcription factor family have been identified. These NF-box binding factors represent novel NF signaling components probably involved in the fine-tuning of NF-mediated expression of host genes.

Contact : Marc Lepetit

Contacts IBIP :
Sabine Zimmermann
Marc Lepetit
Corinne Dasen
Chantal Baracco