Séminaire IBIP
Les séminaires ont lieu sur le Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)

Jeudi 23 octobre 2008
Amphi 208 (Cœur d’Ecole) à 14h

The plastidial methionine sulfoxide reductases B from Arabidopsis thaliana: biochemical characterization and physiological functions

Edith Laugier
BPMP – équipe Intégration

Plants are frequently exposed to abiotic constraints inducing metabolic changes at the expense of growth. The chloroplast, where photosynthesis takes place, is particularly subjected to metabolic disruption resulting in oxidative damage in macromolecules. In proteins, sulphur-containing residues like methionine (Met), are the most sensitive to oxidative lesions. Met oxidation to methionine sulfoxide (MetSO) is reversed by two types of methionine sulfoxide reductases (MSRs), A and B, specific to S- and R-enantiomers, respectively. Using a biochemical approach, we identified the reductants for the two plastidial MSRB1 and MSRB2 enzymes. Compared to other organisms, plants display numerous thioredoxins (Trxs) and glutaredoxins (Grxs) that are likely to play this function.. We found that MSRB1 and MSRB2 possess distinct electron donors among the various types of Trxs and Grxs. To assess the physiological role of plastidial MSRBs, we characterized Arabidopsis mutant lines knockout for one gene or both of them, and lines overexpressing MSRB1 or MSRB2. Knockout and overexpressing plants display significantly decreased and increased MSR activities, respectively. No phenotype was noticed for all plant types under optimal growing conditions. Under high light or low temperature treatments, plants knockout for both MSRB1 and MSRB2 exhibit a substantial delay in growth. These data show the importance of the repair mechanisms of oxidized Met in the plant responses to environmental constraints.

Contact : Marc Lepetit

Contacts IBIP :
Sabine Zimmermann
Marc Lepetit
Corinne Dasen
Chantal Baracco