Institut des Sciences des Plantes de Montpellier (IPSiM)

Belgaroui N, Berthomieu P, Rouached H, Hanin M (2016) The secretion of the bacterial phytase PHY-US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth. Plant Biotechnol. J., 14(9):1914-1924

Phytic acid (PA) is a major source of inorganic phosphate (Pi) in the soil. Plants are however unable to use PA for Pi nutrition and growth. Arabidopsis was engineered to secrete a bacterial phytase. Compared to the control, it showed normal growth and no hallmark of Pi deficiency when grown on phytic acid as sole P source. Interestingly, co-cultivation of the engineered plants with controls showed that the secreted phytases benefitted to the neighbouring control plants, which showed no sign of Pi deficiency and normal growth.

The work was done in collaboration with the Centre de Biotechnologie de Sfax in Tunisia.

Auguy F, Fahr M, Moulin P, El Mzibri M, Smouni A, Filali-Maltouf A, Béna G, Doumas P✉ (2016) Transcriptome changes in Hirschfeldia incana in response to lead exposure. Front. Plant Sci., 6:1231

Belgaroui N, Berthomieu P, Rouached H✉, Hanin M✉ (2016) The secretion of the bacterial phytase PHY-US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth. Plant Biotechnol. J., 14(9):1914-1924

Bouain N, Doumas P, Rouached H✉ (2016) Recent advances in understanding the molecular mechanisms regulating the root system response to phosphate deficiency in Arabidopsis. Curr. Genomics, 17(4):308-314

Doumas P✉, Munoz M, Banni M, Becerra S, Bruneel O, Casiot C, Cleyet-Marel J-C, Gardon J, Noack Y, Sappin-Didier V (2017) Polymetallic pollution from abandoned mines in Mediterranean regions: a multidisciplinary approach to environmental risks. Reg. Environ. Change, (in press)

Kozhevnikova AD✉, Seregin IV, Gosti F, Schat H (2017) Zinc accumulation and distribution over tissues in Noccaea caerulescens in nature and in hydroponics: a comparison. Plant Soil, 411(1):5-16

Saenchai C*, Bouain N*, Kisko M, Prom-U-Thai C, Doumas P, Rouached H✉ (2016) The involvement of OsPHO1;1 in the regulation of iron transport through integration of phosphate and zinc deficiency signalling. Front. Plant Sci., 7:396

Saenchai C, Prom-U-Thai C✉, Lordkaew S, Rouached H, Rerkasem B (2016) Distribution of iron and zinc in plant and grain of different rice genotypes grown under aerobic and wetland conditions. J. Cereal. Sci., 71:108-115

Fahr M, Laplaze L, El Mzibri M, Doumas P, Bendaou N, Hocher V, Bogusz D, Smouni A✉ (2015) Assessment of lead tolerance and accumulation in metallicolous and non-metallicolous populations of Hirschfeldia incana. Environ. Exp. Bot., 109:186-192

Kisko M*, Bouain N*, Rouached A*, Choudhary SP, Rouached H✉ (2015) Molecular mechanisms of phosphate and zinc signaling crosstalk in plants: Phosphate and zinc loading into root xylem in Arabidopsis. Environ. Exp. Bot., 114:57-64

Mith O, Benhamdi A, Castillo T, Bergé M, MacDiarmid CW, Steffen J, Eide DJ, Perrier V, Subileau M, Gosti F, Berthomieu P, Marquès L✉ (2015) The antifungal plant defensin AhPDF1.1b is a beneficial factor involved in adaptive response to zinc overload when it is expressed in yeast cells. Microbiology Open, 4(3):409-422

Belgaroui N, Zaidi I, Farhat A, Chouayekh H, Bouain N, Chay S, Curie C, Mari S, Masmoudi K, Davidian J-C, Berthomieu P, Rouached H, Hanin M (2014) Overexpression of the bacterial phytase US417 in Arabidopsis reduces the concentration of phytic acid and reveals its involvement in the regulation of sulfate and phosphate homeostasis and signaling. Plant Cell Physiol., 55(11):1912-1924

Bouain N, Kisko M, Rouached A, Dauzat M, Lacombe B, Belgaroui N, Ghnaya T, Davidian J-C, Berthomieu P, Abdelly C, Rouached H (2014) Phosphate/zinc interaction analysis in two lettuce varieties reveals contrasting effects on biomass, photosynthesis, and dynamics of Pi transport. Biomed Res. Int., 2014:548254

Bouain N*, Shahzad Z*, Rouached A*, Khan GA, Berthomieu P, Abdelly C, Poirier Y, Rouached H (2014) Phosphate and zinc transport and signalling in plants: toward a better understanding of their homeostasis interaction. J. Exp. Bot., 65(20):5725-5741

Hermand V, Julio E, Dorlhac de Borne F, Punshon T, Richachenevsky FK, Bellec A, Gosti F, Berthomieu P (2014) Inactivation of two newly identified tobacco heavy metal ATPases leads to reduced Zn and Cd accumulation in shoots and reduced pollen germination. Metallomics, 6(8):1427-1440

Khan GA, Bouraine S, Wege S, Li Y, de Carbonnel M, Berthomieu P, Poirier Y, Rouached H (2014) Coordination between zinc and phosphate homeostasis involves the transcription factor PHR1, the phosphate exporter PHO1, and its homologue PHO1;H3 in Arabidopsis. J. Exp. Bot., 65(3):871-884

Meindre F, Lelièvre D, Loth K, Mith O, Aucagne V, Berthomieu P, Marquès L, Delmas AF, Landon C, Paquet F (2014) The nuclear magnetic resonance solution structure of the synthetic AhPDF1.1b plant defensin evidences the structural feature within the γ-motif. Biochemistry-US, 53(49):7745-7754

Nguyen NNT, Ranwez V, Vile D, Soulié M-C, Dellagi A, Expert D, Gosti F (2014) Evolutionary tinkering of the expression of PDF1s suggests their joint effect on zinc tolerance and the response to pathogen attack. Front. Plant Sci., 5:70

Auguy F, Fahr M, Moulin P, Brugel A, Laplaze L, El Mzibri M, Filali-Maltouf A, Doumas P, Smouni A (2013) Lead tolerance and accumulation in Hirschfeldia incana, a Mediterranean Brassicaceae from metalliferous mine spoils . PLoS one, 8(5):e61932

Rouached H (2013) Recent developments in plant zinc homeostasis and the path toward improved biofortification and phytoremediation programs. Plant Signal. Behav., 8(1):e22681

Shahzad Z, Ranwez V, Fizames C, Marquès L, Le Martret B, Alassimone J, Godé C, Lacombe E, Castillo T, Saumitou-Laprade P, Berthomieu P, Gosti F (2013) Plant Defensin type 1 (PDF1): protein promiscuity and expression variation within the Arabidopsis genus shed light on zinc tolerance acquisition in Arabidopsis halleri. New Phytol., 200(3):820-833