Doctorate thesis of Montpellier University

Friday december 10 at 10 am, Amphi Lamour


Functional characterization of Arabidopsis BolA proteins in iron-sulfur center transfer and iron homeostasis

Ecole Doctorale : GAIA – Biodiversité, Agriculture, Alimentation, Environnement, Terre, Eau
Spécialité : BIDAP – Biologie, Interactions, Diversité Adaptative des Plantes
Etablissement : L’institut Agro – Montpellier SupAgro
Unité de recherche : UMR 5004 – BPMP – Biochimie et Physiologie Moléculaire des Plantes



Sandrine Ollagnier de Choudens – Directeur de recherche au laboratoire Chimie et Biologie des Métaux – Rapportrice

Jean-Phillipe Reicheld – Directeur de recherche au Laboratoire Génome et Développement des Plantes – Rapporteur

Louis Grillet – Professeur assistant au National Taiwan University – Examinateur

Sébastien Besteiro – Directeur de recherche au Laboratory of Pathogen Host Interaction – Examinateur

Pierre Czerni – Professeur eu Plant Health Institute Montpellier – Examinateur

Florence Vignols – CR CNRS – Directrice de thèse


Team: Mineral nutrition and oxidative stress




Iron is a microelement crucial for the function of a large number of biological way.

One of the iron assimilation products is the iron sulphur (Fe-S) cluster. These cofactors are implicated in various essential reaction like photosynthesis or mitochondrial respiration. A large amount of protein’s families is involved in the Fe-S proteins maturation, among them, there is the BOLA family. My research focus on the function of two member of the BOLA family in plants called BOLA1 and BOLA4. By transcriptomic approach and transgenic lines study with rapporteur genes, I could show that BOLA1 and BOLA4 genes have a constitutive expression profile and there are presents in both plastids and mitochondria. After the creation and characterization of the bola1 bola4 double-mutant, I demonstrated that BOLA1 and BOLA4 proteins have a functional redundancy and are very important in plant growth. The phenotypic characterization, coupled with proteomic approach, showed that BOLA1 and BOLA4 are involved in the root architecture maintenance, long-distance signalling, carbon assimilation and iron homeostasis regulation. It was also observed that BOLA1 and BOLA4 are implicated in the maintenance of many biological ways involving Fe-S cluster proteins. These results bring a better comprehension of the functions of BOLA family and describe for the first time a role of BOLA1 and BOLA4 in plants.