Tuesday septembre 14 2021 at 2 pm
(University of Washington, Seattle)
Decoding and recoding plant development using integrase switches
There are many open questions about the molecular mechanisms that coordinate the dynamic, multicellular behaviors required for organogenesis. By building synthetic circuits to either record or re-code in vivo signaling networks, synthetic biology brings potential novel inroads into studies of development. Serine integrases mediating site-specific and irreversible DNA recombination allow the implementation of history-dependent logic circuits. By developing an in vivo temporal tracker using recombinases, we will track and decode the cell-lineage gene-expression of signaling events involved in lateral root development in the reference plant Arabidopsis thaliana. To date, using promoters active during early lateral root development, we obtained the first integrase switch specific to the early stage of lateral root development. We are also using integrase switches to tune the development of lateral root development by inducing transcription factors in synthetic integrase-based circuits. The techniques developed here should serve as a proof-of-principle that can be extended to studying the development of other organs, as well as to developmental studies in diverse organisms.
Contact: Alexis Deangeli