Campus Montpellier SupAgro/INRA de La Gaillarde (2, place P. Viala Montpellier)
Thursday 12 september 2019 Room 106 (School Heart) at 14:00
School of Plant and Environmental Sciences Virginia Tech Blacksburg, VA
Plant Protoplasts: a Means to an End & a New Beginning
Plant protoplasts are both a versatile tool, e.g. for the study of transcriptional networks, and a starting point for plant regeneration, e.g. for the purposes of crop trait improvement through new plant breeding technologies (NPBT). The release of single cells from their fixed position in a plant organ by the enzymatic digestion of the cell wall allows us to separately analyze characteristics of distinct cell types. This can be achieved through fluorescence-activated cell-sorting of GFP-marker lines that express in specific cell lineages. This technique was applied to study the transcriptomic responses to auxin treatment in the Arabidopsis root and the data was further analyzed in relation to datasets of spatial-expression and developmental regulation. Furthermore, transient transformation of protoplasts can be utilized to rapidly analyze the transcriptional networks downstream of a signaling cascade or transcription factor of interest and methods have been developed for the cytometric isolation of successfully transformed cells and the determination of direct transcriptional targets. This technique allows for swift, high-throughput analysis and opens up possibilities for study in various species. Lastly, transient transformation of protoplasts with gene-editing tools and subsequent regeneration of single cells into true-to-type plants makes possible the non-transgenic manipulation of the plant genome which, in some parts of the world, is considered to be non-GMO. This NPBT was used to engineer herbicide tolerance in a farmer-preferred cassava cultivar as part of a Bill and Melinda Gates Foundation-funded project.
Contact: Gabriel Krouk