IBIP seminar

Friday, March 17, 2017

Histological and biochemical traits involved in the biomass degradability in maize. Genetic determinism and drought impact

Fadi El Hage
Institut Jean-Pierre Bourgin – INRA Versailles-Grignon

Maize stem histological pattern variation and its impact on lignocellulosic biomass degradability is the main focus here. The purpose of this study is to highlight key histological parameters involved in the degradability in addition to the variation explained by biochemical variation. To do so, internodes from a set of 11 contrasted lines have been harvested on field trials carried out over the 3 last years.
In the context of sustainable and dedicated crop for biomass production for bio-refinery or animal feeding valorization, water supply will be constrained. Internodes from plants cultivated in water deficit regime have also been harvested to get insights into the responses of histological and biochemical parameters to drought.
QTL detection will be performed on a new recombinant inbred lines population to depict the genetic determinism of all the studied traits. A dense genetic map (1000 SNP) is already available for this population and internodes from each line have been harvested on plant cultivated under contrasted water regimes.
Finally, the characterization of cell wall compounds under a drought stress will be realized thanks to the analysis of enzymatic digestions on histological sections from the panel individuals or the recombinant inbred lines population. In addition, biochemical analysis of micro-dissection from different tissue type will lead to a better understanding of the internode histological pattern answer to the drought stress.

Contact : Arthur Poitout

Contacts IBIP :
Sabine Zimmermann
Alexandre Martiniere
Christine Granier
Chantal Baracco