Electrophysiology of plant mineral nutrition and root symbioses
Group leader : Hervé Sentenac
Research Director INRAE
Key words
membrane transport, patch-clamp, spectrofluorimetry, potassium, nitrate, salt stress, water stress, stomata
Presentation
Our group is interested in the role of ion channels and ion transporters in mineral nutrition and interactions of the plant with symbiotic microorganisms (ectomycorrhizae, nitrogen-fixing rhizobia, plant growth promoting rhizobacteria).
The model organisms are the ectomycorrhizal fungus Hebeloma cylindrosporum in interaction with the pine Pinus pinaster, the legume Medicago truncatula in interaction with nitrogen-fixing rhizobia Sinorhizobium melilotti, and the model plant Arabidopsis thaliana in interaction with plant growth promoting rhizobacteria.
The developed strategies include molecular and cell biology, reverse genetics, electrophysiology and whole plant biology.
The objective is to describe physiological functions and roles of the involved transport systems and to get an integrative view of their contribution to the plant mineral nutrition, root symbioses and adaptation to environmental conditions.
Team members
Main results
Significant publications
Drain A*, Thouin J*, Wang L-M, Boeglin M, Pauly N, Nieves-Cordones M, Gaillard I, Véry A-A, Sentenac H✉ (2020) Functional characterization and physiological roles of the single Shaker outward K+ channel in Medicago truncatula. Plant J., 102(6):1249-1265
Garriga M✉, Raddatz N, Véry A-A, Sentenac H, Rubio-Meléndez ME, González W, Dreyer I✉ (2017) Cloning and functional characterization of HKT1 and AKT1 genes of Fragariaspp. – relationship to plant response to salt stress. J. Plant Physiol., 210:9-17
Charpentier M✉, Sun J, Vaz Martins T, Radhakrishnan GV, Findlay K, Soumpourou E, Thouin J, Véry A-A, Sander D, Morris RJ, Oldroyd GED✉ (2016) Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic calcium oscillations. Science, 352(6289):1102-1105
Damiani I*, Drain A*, Guichard M, Balzergue S, Boscari A, Boyer J-C, Brunaud V, Cottaz S, Rancurel C, Da Rocha M, Fizames C, Fort S, Gaillard I, Maillol V, Danchin EGJ, Rouached H, Samain E, Su Y-H, Thouin J, Touraine B, Puppo A, Frachisse J-M, Pauly N✉, Sentenac H✉ (2016) Nod factor effects on root hair-specific transcriptome ofMedicago truncatula: Focus on plasma membrane transport systems and reactive oxygen species networks. Front. Plant Sci., 7:794
Garcia K*, Doidy J*, Zimmermann S, Wipf D, Courty P-E✉ (2016) Take a trip through the plant and fungal transportome of Mycorrhiza. Trends Plant Sci., 21(11):937-950
Wang L, Yang S-Y, Guo M-Y, Huang Y-N, Sentenac H, Véry A-A✉, Su Y-H✉ (2016) The S1-S2 linker determines the distinct pH sensitivity between ZmK2.1 and KAT1. Plant J., 85(5):675-685
Doré J, Perraud M, Dieryckx C, Kohler A, Morin E, Henrissat B, Lindquist EA, Zimmermann S, Girard V, Kuo A, Grigoriev IV, Martin F, Marmeisse R, Gay G✉ (2015) Comparative genomics, proteomics and transcriptomics give new insight into the exoproteome of the basidiomycete Hebeloma cylindrosporum and its involvement in ectomycorrhizal symbiosis. New Phytol., 208(4):1169-1187
Taochy C, Gaillard I, Ipotesi E, Oomen RJFJ, Leonhardt N, Zimmermann S, Peltier J-B, Szponarski W, Simonneau T, Sentenac H, Gibrat R, Boyer J-C✉ (2015) The Arabidopsisroot stele transporter NPF2.3 contributes to nitrate translocation to shoots under salt stress. Plant J., 83(3):466-479
Yang G, Sentenac H, Véry A-A, Su Y-H✉ (2015) Complex interactions among residues within pore region determine the K+ dependence of a KAT1-type potassium channelAmKAT1. Plant J., 83(3):401-412
Farissi M, Faghire M, Bargaz A, Bouizgaren A, Makoudi B, Sentenac H, Ghoulam C✉ (2014) Growth, nutrients concentrations, and enzymes involved in plants nutrition of Alfalfa populations under saline conditions. J. Agr. Sci. Tech., 16(2):301-314
Garcia K, Delteil A, Conéjéro G, Becquer A, Plassard C, Sentenac H, Zimmermann S✉ (2014) Potassium nutrition of ectomycorrhizal Pinus pinaster: overexpression of the Hebeloma cylindrosporum HcTRK1 transporter affects the translocation of both K+ and phosphorus in the host plant. New Phytol., 201(3):951-960
Garcia K, Zimmermann S✉ (2014) The role of mycorrhizal associations in plant potassium nutrition. Front. Plant Sci., 5:337
Collaborations
Funding
Former team members
