Heterologous expression and plant electrophysiology

IPSiM hosts a Heterologous Expression and Plant Electrophysiology platform (EHEV) unique in France. The platform brings together 8 recording setups dedicated to specific applications for heterologous expression of plant membrane proteins in cellular expression systems (xenopus oocytes, animal cells, plant protoplasts), and / or for electrophysiological analyses (characterization of the activity of transport proteins, mainly by voltage-clamp or patch-clamp on isolated cells). It is placed under the scientific responsibility of Anne-Aliénor Véry, DR CNRS and the engineer in charge of the platform is Claire Corratgé-Faillie, IR CNRS.
The platform also serves as a support for theoretical and practical trainings of students within the framework of thematic schools (“Transport”, Univ. Montpellier, Montpellier School of electrophysiology “E2M”, CNRS / INSERM / Univ. Montpellier and the biennal International School “Mistral”) and each year trains young researchers in electrophysiology.

Please clearly mention the advice and technical support provided by the platform staff either by adding the staff directly involved in the list of co-authors or by mentioning the platform in the acknowledgments:
Example : « We acknowledge the Heterologous Expression and Plant Electrophysiology (EHEV) platform from Institute for Plant Sciences of Montpellier (IPSiM) for their advices and assistance. »

Staff / Organizational chart
Materials / Technologies

Justyna Jaślan, Alexis De Angeli (2022) Heterologous expression reveals that GABA does not directly inhibit the vacuolar anion channel AtALMT9. Plant Physiol. (accepted) [doi] [Abstract] [pdf] [PubMed]

Wang L, Ning Y, Sun J, Wilkins KA, Matthus E, McNelly RE, Dark A, Rubio L, Moeder W, Yoshioka K, Véry A-A, Stacey G, Leblanc-Fournier N, Legué V, Moulia B, Davies JM (2022) Arabidopsis thaliana Cyclic Nucleotide-Gated Channel2 mediates extracellular ATP signal transduction in root epidermis. New Phytol., 234(2):412-421

Nieves-Cordones M, Azeem F, Long Y, Boeglin M, Duby G, Mouline K, Hosy E, Vavasseur A, Chérel I, Simonneau T, Gaymard F, Leung J, Gaillard I, Thibaud J-B, Véry A-A, Boudaoud A, Sentenac H (2022) Non-autonomous stomatal control by pavement cell turgor via the K+ channel subunit AtKC1 . Plant Cell, (accepted)

Wang L, Ning Y, Sun J, Wilkins KA, Matthus E, McNelly RE, Dark A, Rubio L, Moeder W, Yoshioka K, Véry A-A, Stacey G, Leblanc-Fournier N, Legué V, Moulia B, Davies JM (2022) Arabidopsis thaliana Cyclic Nucleotide-Gated Channel2 mediates extracellular ATP signal transduction in root epidermis. New Phytol., 234(2):412-421

Wang L*, Zhao L-N*, Shah IH, Ramirez DC, Boeglin M, Véry A-A, Sentenac H, Zhang Y-D✉ (2022) Na+ Sensitivity of the KAT2-Like Channel Is a Common Feature of Cucurbits and Depends on the S5-P-S6 Segment. Plant Cell Physiol., 63(2):279-289

Hoang MTT, Almeida DM, Chay S, Alcon C, Corratgé-Faillie C, Curie C, Mari S (2021) AtDTX25, a member of the multidrug and toxic compound extrusion family, is a vacuolar ascorbate transporter that controls intracellular iron cycling in Arabidopsis. New Phytol., 231(5):1956-1967

Ronzier E, Corratgé-Faillie C, Sanchez F, Brière C, Xiong TC (2021) Ca2+-dependent protein kinase 6 enhances KAT2 shaker channel activity in Arabidopsis thaliana. Int. J. Mol. Sci., 22(4):1596

Morales de los Ríos L, Corratgé-Faillie C, Raddatz N, Mendoza I, Lindahl M, De Angeli A, Lacombe B, Quintero FJ, Pardo JM (2021) The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23. Plant Physiol. Bioch., 168:239-251

Barbi F, Vallon L, Guerrero-Galán C, Zimmermann SD, Melayah D, Abrouk D, Doré J, Lemaire M, Fraissinet-Tachet L, Luis P, Marmeisse R (2021) Datamining and functional environmental genomics reassess the phylogenetics and functional diversity of fungal monosaccharide transporters. Appl. Microbiol. Biotechnol., 105(2):647-660

Müller T, Neuhäuser B, Ludewig U, Houdinet G, Zimmermann SD, Courty P-E, Wipf D (2020) New insights into HcPTR2A and HcPTR2B, two high-affinity peptide transporters from the ectomycorrhizal model fungus Hebeloma cylindrosporum. Mycorrhiza, 30(6):735-747

Khan I, Mohamed S, Regnault T, Mieulet D, Guiderdoni E, Sentenac H, Véry A-A (2020) Constitutive contribution by the rice OsHKT1;4 Na+ transporter to xylem sap desalinization and low Na+ accumulation in young leaves under low as high external Na+ conditions. Front. Plant Sci., 11:1130

Somasundaram S*, Véry A-A*, Vinekar RS*, Ishikawa T, Kumari K, Pulipati S, Kumaresan K, Corratgé-Faillie C, Sowdhamini R, Parida A, Shabala L, Shabala S, Venkataraman G (2020) Homology modeling identifies crucial amino-acid residues that confer higher Na+ transport capacity of OcHKT1;5 from Oryza coarctata Roxb . Plant Cell Physiol., 61(7):1321-1334

Drain A*, Thouin J*, Wang L-M, Boeglin M, Pauly N, Nieves-Cordones M, Gaillard I, Véry A-A, Sentenac H (2020) Functional characterization and physiological roles of the single Shaker outward K+ channel in Medicago truncatula. Plant J., 102(6):1249-1265

Léran S*, Noguéro M*, Corratgé-Faillie C, Boursiac Y, Brachet C, Lacombe B (2020) Functional characterization of the Arabidopsis abscisic acid transporters NPF4.5 and NPF4.6 in Xenopus oocytes. Front. Plant Sci., 11:144

Hmidi D, Messedi D, Corratgé-Faillie C, Marhuenda T, Fizames C, Zorrig W, Abdelly C, Sentenac H, Véry A-A (2019) Investigation of Na+ and K+ transport in halophytes: Functional analysis of the HmHKT2;1 transporter from Hordeum maritimum and expression under saline conditions. Plant Cell Physiol., 60(11):2423-2435

Locascio A, Marques MC, García-Martínez G, Corratgé-Faillie C, Andrés-Colás N, Rubio L, Fernández JA, Véry A-A, Mulet JM, Yenush LP (2019) BCL2-ASSOCIATED ATHANOGENE4 regulates the KAT1 potassium channel and controls stomatal movement. Plant Physiol., 181(3):1277-1294

Villette J, Cuéllar T, Zimmermann SD, Verdeil J-L, Gaillard I (2019) Unique features of the grapevine VvK5.1 channel support novel functions for outward K+ channels in plants. J. Exp. Bot., 70(21):6181-6193

Thouin J, Guo M-Y, Zribi I, Pauly N, Mouradi M, Cherki G, Sentenac H, Véry A-A (2019) The Medicago truncatula HKT family: Ion transport properties and regulation of expression upon abiotic stresses and symbiosis. bioRxiv,

Sol S, Valkov VT, Rogato A, Noguéro M, Gargiulo L, Mele G, Lacombe B, Chiurazzi M (2019) Disruption of the Lotus japonicus transporter LjNPF2.9 increases shoot biomass and nitrate content without affecting symbiotic performances. BMC Plant Biol., 19:380

Wang L-M, Guo M-Y, Thibaud J-B, Véry A-A, Sentenac H (2019) A repertoire of cationic and anionic conductances at the plasma membrane of Medicago truncatula root hairs. Plant J., 98(3):418-433

Nieves-Cordones M, Andrianteranagna M, Cuéllar T, Chérel I, Gibrat R, Boeglin M, Moreau B, Paris N, Verdeil J-L, Zimmermann SD, Gaillard I (2019) Characterization of the grapevine Shaker K+ channel VvK3.1 supports its function in massive potassium fluxes necessary for berry potassium loading and pulvinus-actuated leaf movements. New Phytol., 222(1):286-300

Prado K, Cotelle V, Li G, Bellati J, Tang N, Tournaire-Roux C, Martinière A, Santoni V, Maurel C (2019) Oscillating aquaporin phosphorylations and 14-3-3 proteins mediate the circadian regulation of leaf hydraulics. Plant Cell, 31(2):417-429

Huang L-T*, Zhao L-N*, Gao L-W, Véry A-A, Sentenac H, Zhang Y-D (2018) Constitutive expression of CmSKOR, an outward K+ channel gene from melon, in Arabidopsis thaliana involved in saline tolerance. Plant Sci., 274:492-502

Guerrero-Galán C, Delteil A, Garcia K, Houdinet G, Conéjéro G, Gaillard I, Sentenac H, Zimmermann SD (2018) Plant potassium nutrition in ectomycorrhizal symbiosis: properties and roles of the three fungal TOK potassium channels in Hebeloma cylindrosporum. Environ. Microbiol., 20(5):1873-1887

Noguéro M*, Léran S*, Bouguyon E, Brachet C, Tillard P, Nacry P, Gojon A, Krouk G, Lacombe B (2018) Revisiting the functional properties of NPF6.3/NRT1.1/CHL1 in Xenopus oocytes. bioRxiv,

Nieves-Cordones M, Mohamed S, Tanoi K, Kobayashi N, Takagi K, Vernet A, Guiderdoni E, Périn C, Sentenac H, Véry A-A (2017) Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system. Plant J., 92(1):43-56

Corratgé-Faillie C, Ronzier E, Sanchez F, Prado K, Kim J-H, Lanciano S, Leonhardt N, Lacombe B, Xiong TC (2017) The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33. Febs Lett., 591(13):1982-1992

Campbell MT, Bandillo N, Al Shiblawi FRA, Sharma S, Liu K, Du Q, Schmitz AJ, Zhang C, Véry A-A, Lorenz AJ, Walia H (2017) Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content. PLoS Genet., 13(6):e1006823

Tounsi S, Ben Amar S, Masmoudi K, Sentenac H, Brini F, Véry A-A (2016) Characterisation of two HKT1;4 transporters from Triticum monococcum to elucidate the determinants of the wheat salt tolerance Nax1 QTL. Plant Cell Physiol., 57(10):2047-2057

Charpentier M, Sun J, Vaz Martins T, Radhakrishnan GV, Findlay K, Soumpourou E, Thouin J, Véry A-A, Sander D, Morris RJ, Oldroyd GED (2016) Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic calcium oscillations. Science, 352(6289):1102-1105

Wang L, Yang S-Y, Guo M-Y, Huang Y-N, Sentenac H, Véry A-A, Su Y-H (2016) The S1-S2 linker determines the distinct pH sensitivity between ZmK2.1 and KAT1. Plant J., 85(5):675-685

Lefoulon C*, Boeglin M*, Moreau B, Véry A-A, Szponarski W, Dauzat M, Michard E, Gaillard I, Chérel I (2016) The Arabidopsis AtPP2CA protein phosphatase inhibits the GORK K+ efflux channel and exerts a dominant suppressive effect on phosphomimetic-activating mutations. J. Biol. Chem., 291(12):6521-6533

Yang G, Sentenac H, Véry A-A, Su Y-H (2015) Complex interactions among residues within pore region determine the K+ dependence of a KAT1-type potassium channel AmKAT1. Plant J., 83(3):401-412

Grondin A, Rodrigues O, Verdoucq L, Merlot S, Leonhardt N, Maurel C (2015) Aquaporins contribute to ABA-triggered stomatal closure through OST1-mediated phosphorylation. Plant Cell, 27(7):1945-1954

Léran S, Edel KH, Pervent M, Hashimoto K, Corratgé-Faillie C, Offenborn JN, Tillard P, Gojon A, Kudla J, Lacombe B (2015) Nitrate sensing and uptake in Arabidopsis are enhanced by ABI2, a phosphatase inactivated by the stress hormone abscisic acid. Sci. Signal., 8(375):ra43

Léran S, Garg B, Boursiac Y, Corratgé-Faillie C, Brachet C, Tillard P, Gojon A, Lacombe B (2015) AtNPF5.5, a nitrate transporter affecting nitrogen accumulation in Arabidopsis embryo. Sci. Rep.-UK, 5:7962

Ronzier E, Corratgé-Faillie C, Sanchez F, Prado K, Brière C, Leonhardt N, Thibaud J-B, Xiong TC (2014) CPK13, a non-canonical CPK, specifically inhibits KAT2 and KAT1 Shaker channels and reduces stomatal opening. Plant Physiol., 166(1):314-326

Jammes F, Leonhardt N, Tran D, Bousserouel H, Véry A-A, Renou J-P, Vavasseur A, Kwak JM, Sentenac H, Bouteau F, Leung J (2014) Acetylated 1,3-diaminopropane antagonizes abscisic acid-mediated stomatal closing in Arabidopsis. Plant J., 79(2):322-333

Nieves-Cordones M, Chavanieu A, Jeanguenin L, Alcon C, Szponarski W, Estaran S, Chérel I, Zimmermann S, Sentenac H, Gaillard I (2014) Distinct amino acids in the C-linker domain of the Arabidopsis K+ channel KAT2 determine its subcellular localization and activity at the plasma membrane. Plant Physiol., 164(3):1415-1429

Xiong TC, Ronzier E, Sanchez F, Corratgé-Faillie C, Mazars C, Thibaud J-B (2014) Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter. Front. Plant Sci., 5:43

Ben Amar S, Brini F, Sentenac H, Masmoudi K, Véry A-A (2014) Functional characterization in Xenopus oocytes of Na+ transport systems from durum wheat reveals diversity among two HKT1;4 transporters. J. Exp. Bot., 65(1):213-222

Ranocha P, Dima O, Nagy R, Felten J, Corratgé-Faillie C, Novák O, Morreel K, Lacombe B, Martinez Y, Pfrunder S, Jin X, Renou J-P, Thibaud J-B, Ljung K, Fischer U, Martinoia E, Boerjan W, Goffner D (2013) Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis. Nat. Commun., 4:2625

Cuéllar T, Azeem F, Andrianteranagna M, Pascaud F, Verdeil J-L, Sentenac H, Zimmermann S, Gaillard I (2013) Potassium transport in developing fleshy fruits: The grapevine inward K+ channel VvK1.2 is activated by CIPK-CBL complexes and induced in ripening berry flesh cells. Plant J., 73(6):1006-1018

Sassi A, Mieulet D, Khan I, Moreau B, Gaillard I, Sentenac H, Véry A-A (2012) The rice monovalent cation transporter OsHKT2;4: revisited ionic selectivity. Plant Physiol., 160(1):498-510

Oomen RJFJ, Benito B, Sentenac H, Rodríguez-Navarro A, Talón M, Véry A-A, Domingo C (2012) HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism. Plant J., 71(5):750-762

Laohavisit A*, Shang Z*, Rubio L, Cuin TA, Véry A-A, Wang A, Mortimer JC, Macpherson N, Coxon KM, Battey NH, Brownlee C, Park OK, Sentenac H, Shabala S, Webb AAR, Davies JM (2012) Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+– and K+-permeable conductance in root cells. Plant Cell, 24(4):1522-1533

Lopez D, Bronner G, Brunel N, Auguin D, Bourgerie S, Brignolas F, Carpin S, Tournaire-Roux C, Maurel C, Fumanal B, Martin F, Sakr S, Label P, Julien J-L, Gousset-Dupont A, Venisse J-S (2012) Insights into Populus XIP aquaporins: evolutionary expansion, protein functionality, and environmental regulation. J. Exp. Bot., 63(5):2217-2230

Mian A*, Oomen RJFJ*, Isayenkov S, Sentenac H, Maathuis FJM, Véry A-A (2011) Overexpression of a Na+ and K+-permeable HKT transporter in barley improves salt tolerance. Plant J., 68(3):468-479

Jeanguenin L, Alcon C, Duby G, Boeglin M, Chérel I, Gaillard I, Zimmermann S, Sentenac H, Véry A-A (2011) AtKC1 is a general modulator of Arabidopsis inward Shaker channel activity. Plant J., 67(4):570-582

Held K*, Pascaud F*, Eckert C, Gajdanowicz P, Hashimoto K, Corratgé-Faillie C, Offenborn JN, Lacombe B, Dreyer I, Thibaud J-B, Kudla J (2011) Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/CIPK6 calcium sensor/protein kinase complex. Cell Res., 21(7):1116-1130

Zhang Y-D*, Véry A-A*, Wang L-M, Deng Y-W, Sentenac H, Huang D-F (2011) A K+ channel from salt-tolerant melon inhibited by Na+. New Phytol., 189(3):856-868

Gajdanowicz P, Michard E, Sandmann M, Rocha M, Guedes Corrêa LG, Ramírez Aguilar S, Gomez-Porras JL, González W, Thibaud J-B, van Dongen JT, Dreyer I (2011) Potassium (K+) gradients serve as a mobile energy source in plant vascular tissues. P. Natl. Acad. Sci. USA, 108(2):864-869




July 2-13, 2018 – Montpellier International School on ion and water TRAnsport in PLant

E2M 2018

March 26-30 2018 (theorie) & May 14-18 (practice) – Ecole d’électrophysiologie de Montpellier – Contact: Hervé sentenac