Equipment :
Orbitrap ExplorisTM 240 Mass Spectrometer (Thermo Fisher Scientific)
Technical description :
The Exploris 240 is a benchtop mass spectrometer for precursor ion selection by a high performance quadrupole and detection by an Orbitrap Fourier transform analyzer. It is a high resolution, high mass accuracy, high sensitivity mass spectrometer. It is particularly suitable:
- work on identifying scarce proteins in very complex mixtures
- targeted quantification approaches
- expression analyzes with or without labeling in very complex mixtures
- identification and quantification approaches in the same analysis
- high resolution MS / MS experiments such as De Novo sequencing, whole protein analysis
2 sources are available:
- The H ESI II probe which is used to calibrate the instrument
- The Nano ESI (Nanospray Flex) source is used in conjunction with the nano-LC-MS / MS
Technical performances :
- Mass range : from 40 to 6000 m/z
- Resolution of 240,000 at m/z 200
- Achieve sub 1-ppm mass accuracy levels with Thermo Scientific EASY-IC internal calibration source and single mass calibration for full mass range
- Scan rates: Up to 22 Hz at resolution setting of 15,000 at m/z 200