Equipment :

 

Orbitrap ExplorisTM 240 Mass Spectrometer (Thermo Fisher Scientific)

 

Technical description :

 

The Exploris 240 is a benchtop mass spectrometer for precursor ion selection by a high performance quadrupole and detection by an Orbitrap Fourier transform analyzer. It is a high resolution, high mass accuracy, high sensitivity mass spectrometer. It is particularly suitable:

  • work on identifying scarce proteins in very complex mixtures
  • targeted quantification approaches
  • expression analyzes with or without labeling in very complex mixtures
  • identification and quantification approaches in the same analysis
  • high resolution MS / MS experiments such as De Novo sequencing, whole protein analysis

2 sources are available:

  • The H ESI II probe which is used to calibrate the instrument
  • The Nano ESI (Nanospray Flex) source is used in conjunction with the nano-LC-MS / MS
 
Technical performances :

 

  • Mass range : from 40 to 6000  m/z
  • Resolution of 240,000 at m/z 200
  • Achieve sub 1-ppm mass accuracy levels with Thermo Scientific EASY-IC internal calibration source and single mass calibration for full mass range
  • Scan rates: Up to 22 Hz at resolution setting of 15,000 at m/z 200