Equipment :
High pressure Nano system U3000 (Thermo Fisher Scientific)
Brief description :
A gradient nano-UHPLC tandem chromatography chain for coupling with a Maxis Impact HD mass spectrometer.
Technical description :
The compact and modular nano-HPLC module includes a nano-HPLC analysis pump, a ternary gradient micro-HPLC auxiliary pump for performing the sample loading / desalting phases, a thermostatted enclosure and a degasser.
-Nano-pump FLM-3100 :
High pressure binary gradient pump with flow splitter. This pump supports pressures up to 500 bars. Traditionally used on MSPP platform with 25cm column.
-Micro-pump DGP-3600M :
The system is also equipped with a pump allowing work flows from 10μL / min to 2.5ml / min. This pump allows the samples to be loaded and desalted on concentration cartridges before their nano-HPLC analysis.
– Automatic thermostatted micro-injecteur
3 x micro-plates 96 wells
Injection technology: Two coaxial needles allow the piercing of speta and the taking of samples by flexible needle (allowing the collection of the whole sample, eg sampled in 1μL sample).