Equipment :

High pressure Nano system U-HPLC NCS3500RS (Thermo Fisher Scientific)

Brief description :

Chromatographic system with gradient, online with a Thermo Scientific Q-Exactive Plus Mass Spectrometer

Technical description :

The compact and modular nano-UHPLC module comprises a nano-U-HPLC analysis pump, a ternary gradient micro-HPLC auxiliary pump for performing the sample loading / desalting phases and a thermostated enclosure .

The very high pressure pumping system (up to 900 bar) with a very low gradient delay (25nL in nano mode) and the nanoViper with no dead volume and manual clamping revolutionize the implementation of nanoscale analyses.

The very high resolution columns (sub-2 μm or monolithic particles) and a great length can thus be exploited with efficiency and ease of implementation without equal.

-Nano-pump UHPLC :

Direct-pump (without split) with high-pressure binary gradient pump consisting of independent pumps with two pistons on each of the 2 solvent paths.

This very high pressure pump (900 Bar) has a minimum gradient delay (25nL in Nano mode). Traditionally used on platform with column of 50 cm.

-Micro-pump :

The system is also equipped with a ternary gradient pump 620 bars allowing work flows from 10μL / min to 2.5ml / min. This pump allows the samples to be loaded and desalted on concentration cartridges before their nano-HPLC analysis.

– Automatic thermostatted micro-injector NANO-HPLC WPS-3000TPL

3 x micro-plates 96 wells

Injection technology: Two coaxial needles allow the piercing of speta and the taking of samples by flexible needle (allowing the collection of the whole sample, eg sampled in 1μL sample).