High pressure Nano system U-HPLC NCS3500RS (Thermo Fisher Scientific)
Brief description :
Chromatographic system with gradient, online with a Thermo Scientific Q-Exactive Plus Mass Spectrometer
Technical description :
The compact and modular nano-UHPLC module comprises a nano-U-HPLC analysis pump, a ternary gradient micro-HPLC auxiliary pump for performing the sample loading / desalting phases and a thermostated enclosure .
The very high pressure pumping system (up to 900 bar) with a very low gradient delay (25nL in nano mode) and the nanoViper with no dead volume and manual clamping revolutionize the implementation of nanoscale analyses.
The very high resolution columns (sub-2 μm or monolithic particles) and a great length can thus be exploited with efficiency and ease of implementation without equal.
-Nano-pump UHPLC :
Direct-pump (without split) with high-pressure binary gradient pump consisting of independent pumps with two pistons on each of the 2 solvent paths.
This very high pressure pump (900 Bar) has a minimum gradient delay (25nL in Nano mode). Traditionally used on platform with column of 50 cm.
The system is also equipped with a ternary gradient pump 620 bars allowing work flows from 10μL / min to 2.5ml / min. This pump allows the samples to be loaded and desalted on concentration cartridges before their nano-HPLC analysis.
– Automatic thermostatted micro-injector NANO-HPLC WPS-3000TPL
3 x micro-plates 96 wells
Injection technology: Two coaxial needles allow the piercing of speta and the taking of samples by flexible needle (allowing the collection of the whole sample, eg sampled in 1μL sample).