IBIP seminar

Thursday, December 06, 2018
Room 106 at 2:00 pm


Genotype and abiotic stress shape the root microbiome of maize

Daniel Schachtman
University of Nebraska Lincoln – Department of Agronomy and Horticulture

The Schachtman Lab recently embarked on a series of scientific challenges related to understanding root interaction with soil microbes. The lab is currently focused on two major questions: 1) What are the functions of root microbes in crop plants and 2) How do plants influence their root microbiome? To begin to answer the first question we have conducted a series of culture independent experiments to develop hypotheses and have created a large microbe culture collection to use for hypotheses testing. One culture independent example of how drought and maize genotype shape the root microbiome will be presented along with a brief description of our culture collection. A second project that will be described focuses on the natural chemical diversity in root exudates in maize. Results from the screening of maize lines to identify those that differ in root exudate composition will be presented. In addition several lines that differed in two major root exudates were tested in soil and the rhizosphere microbiome was assessed using 16S amplicon sequencing. The changes in rhizosphere microbial communities in relationship to the exudates will be presented. All of this work is aimed at gaining a full understanding of how crop plants shape their root microbiomes under different environmental conditions and how this eventually alters the composition and function of the root, rhizosphere and soil microbes that may be beneficial to plant growth, development and stress tolerance.

Contact : Hervé Sentenac

Contacts IBIP :
Sabine Zimmermann
Alexandre Martinière
Florent Pantin
Chantal Baracco
Véronique Rafin