General information

Reference : UMR5004-CECABA-029
Workplace : MONTPELLIER
Date of publication : Monday, August 30, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 December 2021
Proportion of work : Full time
Remuneration : Between 2675€ and 3800€ gross / month – depending on experience
Desired level of education : 5-year university degree
Experience required : Indifferent

Missions

Developed since 100 years on the campus, our lab approaches to understand the nitrogen nutrition in plants aims to understand the molecular mechanisms involved in nutrient uptake and signaling. During these last years, our approaches focused on root nutrition. At present, we want to study the physiological and molecular aspect of the plant’s foliar nutrition. The recruited person will be involved in the SNUp-Reg project. This project aims to characterize the physiological and molecular processes involved in the foliar acquisition of nitrogen compounds (nitrate and urea) in the model plant Arabidopsis thaliana. The recruited post-doc will focus on two genome-wide approaches (transcriptomic and GWAS) in order to identify candidate genes involved in Shoot Nitrogen Uptake. She/He will also be in charge of developing the reporter constructs for live imaging on the candidate genes identified. Finally, He/She will contribute to the physiological characterization of urea and nitrate uptake from leaves.

Activities

– Plant culture (hydroponics, soil, in vitro)
– Set up of experimental procedure in plant biology
– Transcriptomic analysis
– Foliar uptake analysis using stable isotopic tracers
– GWAS
– N quantification (Elemental analysis, SPAD)
– Set up of N quantification using SPAD in Arabidopsis thaliana
– Mutant characterization, transgenic lines production
– Gene cloning
– Production and characterisation of reporter lines for live imaging

Skills

The recruited person should have good skills in :
Plant molecular biology, plant physiology and bioinformatics. Experience in GWAS and transcriptomic analysis approaches will be an advantage.
The candidate must have experience in procedure development and data analysis. He/she should love the teamwork but be able to work autonomously.

Work Context

The work will be developed in the B&PMP laboratory in Montpellier, in the team HoNuDe. The laboratory hosts Isotopic Quantification platform (AQUI) and has easy access to the imaging platform (MRI). The work will be partially supported by a technician.

Constraints and risks

No particular constraint, only possible risks linked to the use of classical molecular biology chemicals products.

Additional Information

All the applications that will not be registered via the CNRS job portal will not be studied.