IBIP webinar

Thursday, April 1, 2021
2pm – visioconference (zoom)

Participer à la réunion Zoom : https://zoom.us/j/98857708744?pwd=MEdoU21PZTFpNjNRN1c3Q1FsZXJHdz09

ID de réunion : 988 5770 8744
Code secret : B01w3z

Marie-Edith Chabouté
Institut de biologie moléculaire des plantes, CNRS, Strasbourg

Nuclear mechanical stress responses in plants

As in all living organisms, plant cells are constantly experiencing mechanical stress. Such mechanical stimuli may impact gene expression. However their link with nuclear mechanics was not yet established in plants, notably through the nucleocytoplasmic interface. We are currently studying in a context of an interdisciplinary network the mechanical stress response at the level of the nucleus. We performed rheometry and AFM to understand the physical properties of Arabidopsis root meristem nuclei in the presence or absence of osmotic stress (mannitol). We also use live cell imaging to detect the morphometric response of the nuclei in plants expressing the nuclear envelope SUN1-GFP in relationship with gene expression. This suggests the existence of a link between physical forces, nuclear shape and mechanics, and gene expression in plants.
While microtubules and their regulators are linked to cell division and cell shape their connection to nuclear mechanics is still puzzling. We showed that the small regulatory proteins GIP1 and GIP2 (GCP3 interacting proteins) constitute a hub at the nucleo-cytoplasmic interface to regulate nuclear architecture and their defects leads ultimately to changes in nuclear mechanics and gene expression. To go further in the role of microtubule cytoskeleton on nuclear mechanics, we have developed microfluidic approaches at the single cell level using Arabidopsis root hair as a model.

Contact : Alexandre Martinière

Contacts IBIP :
Sabine Zimmermann (sabine.zimmermann@cnrs.fr)
Alexandre Martiniere (alexandre.martiniere@cnrs.fr)
Florent Pantin (florent.pantin@supagro.fr)
Chantal Baracco (chantal.baracco@inrae.fr)