Ressource "MSPP Plateform"

Véronique Santoni
Supervisor
Tél : 04 99 61 20 20
Mail : veronique.santoni@supagro.inra.fr
Sonia Hem
Technical assistant
Tél : 04 99 61 24 53
Mail : sonia.hem@supagro.inra.fr


 Presentation 


The MSPP platform in the BPMP laboratory is one of the four sites of the Proteomic Pole of Montpellier (PPM). This structure, labeled proteomics platform by GIS IBiSA nationwide, also has the label “Grand Equipment For Technological Evolution and Science” (GePEToS ) in the Languedoc -Roussillon. At the institutional level, MSPP is labeled as a regional proteomic platform by INRA.

MSPP has a high level of instrumentation in mass spectrometry and robotics, as well as the appropriate infrastructure to support projects as classical proteomics (large-scale protein identification) to more specialized levels (large-scale and targeted protein quantification, identification of post- translational modifications).

MSPP is widely open to the community: over the 2009-2013 period, the platform supported more than a hundred projects, half of them outside of the region (France and International) , both through delivery activities and collaborations, including joint response to request for proposals with recruitment of specialized staff. The whole activity has been approved until 2012 to the Quality Assurance ISO 9001:2008 and is currently undergoing certification (in 2014) with an expanded scope to the whole PPM perimeter.

Since its introduction in 1995, the scope of the concept of proteome has experienced successive and profound changes both with regard to the investigation that the technologies implemented levels.
The objective of MSPP is to make available to its internal and external partners a high level of expertise in mass spectrometry in the following fields:
- Protein identification
- Molecular characterization of post-translational modifications
- Protein quantification.

MSPP has two complementary missions which are (i) a technological watch (ii) reception and training. MSPP brings its expertise to the teams for the development of collaborative programs and ensures the transfer of these developments at the platform.





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 Significant publications 


- Di Pietro M, Vialaret J, Li G-W, Hem S, Prado K, Rossignol M, Maurel C, Santoni V. (2013)  Coordinated post-translational responses of aquaporins to abiotic and nutritional stimuli in Arabidopsis roots. Mol. Cell. Proteomics. 12:3886-97.

- Podda A, Checcucci G, Mouhaya W, Centeno D, Rofidal V, Del Carratore R, Luro F, Morillon R, Ollitrault P, Maserti BE. (2013) Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl- accumulation behaviour. J. Plant Physiol. 2013 170:1101-12.

- Prado K, Boursiac Y, Tournaire-Roux C, Monneuse J.M, Postaire O, Da Ines O., Schäffner A.R., Hem S, Santoni V, Maurel C (2013) Regulation of Arabidopsis leaf hydraulics involves light-dependent phosphorylation of aquaporins in veins. Plant Cell 25:1029-1039

- Desoubeaux G, Jourdan ML, Valera L, Jardin B, Hem S, Caille A, Cormier B, Marchand-Adam S, Bailly E, Diot P, Chandenier J. (2013) Proteomic demonstration of the recurrent presence of inter-alpha-inhibitor H4 heavy-chain during aspergillosis induced in an animal model. Int. J. Med Microbiol. 2013. doi: 10.1016/j.ijmm.2013.11.015.

- Joseph M, Ludevid MD, Torrent M, Rofidal V, Tauzin M, Rossignol R, Peltier JB (2012) Proteomic characterisation of endoplasmic reticulum-derived protein bodies in tobacco leaves. BMC Plant Biol. 12:36

- Morin V, Prieto S, Melines S, Hem S, Rossignol M, Lorca T, Espeut J, Morin N, Abrieu A. (2012) CDK-Dependent Potentiation of MPS1 Kinase Activity Is Essential to the Mitotic Checkpoint. Curr. Biol. 22:289-295

- Monneuse JM, Sugano M, Bécue T, Santoni V, Hem S, Rossignol M (2011) Towards the profiling of the Arabidopsis thaliana plasma membrane transportome by targeted proteomics. Proteomics 11:1789-1797


More publications >>


 People 


Permanent staff
Sonia Hem
INRA - IR
Nathalie Berger
INRA - IE
Sylvain Durot
INRA - AI
Valérie Rofidal
INRA - TR
     
Vincent DEMOLOMBE-LIOZU
INRA - TR
     
Non permanent staff
 
Julie Gil
Master 2