Re-description of the female of Kampimodromus langei Wainstein and Arutunjan (Acari: Phytoseiidae) based on normal and abnormal specimens, with the first description of its male

Kampimodromus langei Wainstein and Arutunjan (Acari: Phytoseiidae) is reported for the first time for the Turkish fauna. It is re-described and illustrated, based on normal and abnormal female specimens from material collected from Quercus cerris L. var. cerris (Fagaceae) trees in Samsun Province on the north coast of Turkey. In addition, the male is described for the first time in this study. Our examination of a series of female specimens of K. langei showed no remarkable differences compared to its original description and subsequent redescriptions. However, on both sides of a female specimen, we observed an additional dorsal seta inserted at the location of seta S1 known from other groups of Mesostigmata closely related to phytoseiid mites. The dorsal shield of the abnormal specimen is also illustrated and its setal measurements are provided. To the best of our knowledge of phytoseiids, the extra seta has been reported only for Typhlodromus (Typhlodromus) laurae Wainstein. The possibility of the more rearward location of anterior dorsal setae z6 and s6 which is known for some phytoseiid species, as well as the presence of extra seta/e on other species of phytoseiid mites, are also briefly discussed.

In this study, we report K. langei for the first time for Turkish fauna. It is re-described and illustrated, based on normal and abnormal female specimens. Also, the male is described for the first time in this study.

Material and methods
This study was conducted in 2021 in several remnant oak forests on the Kurupelit campus of Ondokuz Mayis University in Samsun Province, Turkey. Most of the surveys were done periodically in June, July, August, September and October. Leaves of oak trees were inspected with the aid of a 10X hand magnifying lens and those with phytoseiid mites were collected. The collected leaves were wrapped in paper, placed in a labeled plastic bag and stored in an ice-box until transferred to the laboratory. They were then refrigerated until checked for mites under a stereomicroscope. All of the collected mites were stored in 70% alcohol until cleared in lactophenol and mounted on slides in Hoyer's medium. The slides were then kept in an incubator at 55°C for one week. The mites were identified and illustrated by the second author. The permanent slides were examined under an Olympus® CX-41 microscope. Illustrations were prepared by using a U-Da drawing attachment (Camera Lucida). The taxonomic system used followed Chant and McMurtry (2007), and the setal nomenclature used followed Lindquist and Evans (1965), as adapted by Rowell et al. (1978). The dorsal and ventral setal pattern used was that of Chant and Yoshida-Shaul (1989, 1991, 1992. The nomenclature for solenostomes for the dorsal idiosoma follows that of Athias-Henriot (1975). Leg chaetotaxy follows that of Evans (1963). Measurements are given in micrometers and presented as the mean, followed by the range in parentheses. The examined specimens are deposited in the mite collection of the Acarology Laboratory, Cukurova University, Adana, Turkey. Diagnosis -Dorsal setal pattern 10A:8C, one female specimen with extra dorsal seta on both sides, dorsal shield striated, bearing five pairs of large solenostomes (gd1, gd2, gd6, gd8 and gd9). Dorsal setae serrated, except j4, j5, j6, J2, J5 and Z1 smooth, J2 and Z1 with few barbs in some specimens. Peritreme extending to level of seta z2. Sternal shield smooth with three pairs of setae, genital shield smooth. Ventrianal shield smooth with three pairs of preanal setae and one pair of rounded pores. Calyx of spermatheca cup-shaped; atrium small, nodular, attached to calyx without neck. Fixed digit with three teeth, and movable digit with one tooth. Genu II and Genu IV each with eight setae, and tibia IV seven setae. Macroseta StIV present and with blunt tip. Male dorsal shield reticulated and with setae r3 and R1. Male ventrianal shield striated, with three pairs of setae (JV1, JV2 and ZV2), and one specimen had unpaired seta JV3 on right side. Male spermatodactyl L-shaped with slightly developed toe.

Male
Chelicera - (Figure 2C, D). Fixed digit with three teeth, two apical and one well-behind pilus dentilis; movable digit with one tooth; spermatophoral process L-shaped, with toe slightly developed. The left and the right sides of the illustrated specimen, as well as some other

Remarks
Kampimodromus langei was described by Wainstein and Arutunjan (1973) based on the material collected from Quercus sp. (Fagaceae) in Stepanavan, Armenia. Our collection of this species in Turkey represents a new record for the country as previous studies of the genus Kampimodromus did not include K. langei (Ozman and Cobanoglu 2001; Cobanoglu and Ozman 2002; Kasap and Cobanoglu 2009; Yesilayer and Cobanoglu 2011; Doker et al. 2017, 2018. In addition, the male K. langei is described for the first time in this study. Morphological characters and measurements of the current specimens are similar to those of the original description and subsequent re-descriptions (Wainstein and Arutunjan 1973; Ragusa di Chiara and Tsolakis 1994; Tixier et al. 2008; Cargnus et al. 2012; Faraji and Hoekstra 2021. However, a single female specimen showed an abnormality in having an additional dorsal seta on both sides, with all other morphological characters and measurements of the specimen being almost identical to the other specimens examined in this study, as well as the earlier descriptions. To the best of our knowledge, the extra seta inserted at the location of S1 has previously been reported by Kolodochka (2006) for Typhlodromus (Typhlodromus) laurae Arutunjan, but is not known in any other Phytoseiidae species to date (Rowell et al. 1978; Chant and Yoshida-Shaul 1989; Chant and McMurtry 2007; Ferragut et al. 2010. However, it is not known whether the extra seta reported for T. (T.) laurae was paired or unpaired because Kolodochka (2006) illustrated only the right side of the specimen.
This extra seta could be considered seta z6 or s6 because both are already known for some members of the family Phytoseiidae McMurtry 2007; Papadoulis et al. 2009). However, when those setae are present on other phytoseiid species their insertion is clearly anterior to seta R1 (Kreiter et al. 2002; Faraji et al. 2008; Ferragut et al. 2010; Demite et al. 2020. However, the extra setae described in this study were located posterior to R1. In addition, when the podosomal setae z6 and s6 are present in phytoseiids their insertion is clearly anterior to solenostome gd6 McMurtry 2007; Papadoulis et al. 2009) which is not the case in the abnormal specimens reported by Kolodochka (2006) and in this study. Kolodochka (2006) named this seta, SS. Therefore, we have used the same notation and have described this seta as SS.
In addition, an unpaired seta (JV3) was also observed on the ventrianal shield of a male specimen examined in this study. We consider the presence of this unpaired seta to be a variation because it has been already reported for A. nemorivagus Athias-Henriot and N. bicaudus (Wainstein) by Papadoulis et al. (2009).