Many arthropods harbor diverse microbial communities that can shape their physiology, ecology, and interactions with hosts or prey. Although bacterial symbionts of arthropods have been widely studied, mite-associated viruses remain largely unexplored. Here, we examined both viral and bacterial communities in commercially reared predatory mites, asking whether species maintained under similar production conditions share a common microbiome or retain species-specific microbial signatures. We first characterized viral diversity in Phytoseiulus persimilis, a widely used biocontrol agent of spider mites. Previous work identified an active virome dominated by 17 RNA viruses, mostly Picornavirales. Here, we compared two commercial strains: the traditional “Persi-regular” strain and “Persi-plus,” which can successfully develope on the astigmatic mite Carpoglyphus lactis. RNA-seq analysis showed that most dominant viruses were shared between the strains. PCR screening confirmed several viruses in P. persimilis, including one Iflavirus common across commercial and field populations, suggesting stable virus–host associations. To determine whether similar microbial patterns occur across predatory mites, we expanded the analysis to five commercially important species: the phytoseiids Neoseiulus californicus, N. cucumeris, Galendromus occidentalis, and Amblyseius swirskii, together with the soil-dwelling predator Stratiolaelaps scimitus (Laelapidae). Prey mites were included as controls. Microbial communities were characterized using 16S and 18S rRNA amplicon sequencing (QIIME2–DADA2 pipeline) from four pooled samples per species, with 50 individuals each. Across 24 samples, 2,187 ASVs were detected. To focus on dominant bacteria, we retained only ASVs with >1000 reads, yielding 66 ASVs assigned to nine bacterial genera using the SILVA database. Notably, well-known symbionts such as Wolbachia and Cardiniumwere absent from all mite species examined. Despite being closely related and reared under similar conditions, the predatory mites exhibited distinct bacterial communities. N. californicus was consistently dominated by Pandoraea, which reached over 90% relative abundance in all four replicates, whereas N. cucumeris was dominated mainly by Staphylococcus. G. occidentalis showed a mixed community dominated by Corynebacterium and Serratia, while A. swirskii contained a more variable profile. Interestingly, S. scimitus had overall low bacterial presence, suggesting a nearly ‘apo-symbiotic’ nature. Together, the distinct bacterial genera observed among mite species, and the shared persistent viruses detected in P. persimilis lines, suggest that the microbiome of commercially reared predatory mites is shaped more strongly by host species identity than by shared diet or rearing environment. Ongoing virome analyses across these species aim to clarify the role of viruses in predatory mite biology and biocontrol systems.