Agricultural management has been shown to alter soil mesofauna biodiversity. However, the magnitude and direction of these alterations vary among taxonomic groups and under different environmental conditions. This can be attributed to the complexity of these systems, as well as to the lack of fine taxonomic resolution on some groups, particularly Acari. The goal of this work is to better understand how tilling and various amendments affect soil mesofauna community structure and function in two climatically different regions of Washington state in the US. Experimental plots, manipulated for tillage, residue, organic matter input and pH management, each with four replicates in a randomized block design were sampled in May and October 2024 in Pullman (semi-arid, eastern Washington, “PU”) and Mount Vernon (temperate, western Washington, “MV”). Treatments between these two sites are different, but both follow a gradient from least to most “sustainable”. Fauna were extracted in Berlese-Tullgren funnels. Organisms were sorted into three major groups (“Mites”, “Springtails”, “Other”) for initial counts. Mites were later mounted using Hoyer’s and were identified to family using dichotomous keys. The following results are for Spring 2024 only. In total, 28 mite families were found at both sites, with 16 shared families. An NMDS showed that MV and PU mite community compositions were unique. The most prevalent families in PU were Acaridae, Oppiidae, and Pygmephoridae, and in MV were Acaridae, Oppiidae, and Rhodacaridae. Mite density was not significantly different between locations or treatments. In MV, relative abundances showed that evenness increased across a sustainability gradient (Treatment 1-Treatment 3), but family richness remained the same. Richness was almost twice as high in Treatment 4 (perennial meadow) but was proportionally uneven. Functional diversity was also assessed in MV for these mite families; Treatments 1-3 were dominated by predators and generalists (defined as bacterivore/fungivore/plant detritivores), while Treatment 4 was dominated by parasites. In MV, mite diversity does not appear to be affected by treatment within crop fields, though differs from the perennial meadow treatment. Ongoing work focuses on identification of fall mite samples, as well springtails and insects from both sampling periods. Combined, this will provide a more robust view of the soil mesofauna communities in these plots.