Tyrophagus putrescentiae (Schrank, 1781), commonly known as the mold mite, is a major pest of stored products and a significant source of allergens for both humans and animals. It is also a common pest in microbiology laboratories, where it can disrupt research by contaminating cultures and compromising experimental integrity. Efficient laboratory rearing is essential for studying mite biology, ecology, allergen production, and for evaluating control strategies. The selection of an appropriate food source is a key factor in the success of rearing efforts, as nutritional quality directly influences mite development, fecundity, and population growth. This study evaluated ten bacterial species as potential alternative food sources for rearing T. putrescentiae under controlled laboratory conditions, aiming to identify their optimal diet and help us to find a way to prevent their presence in storage facilities and research environments. Understanding the feeding preferences of this pest is critical for developing targeted management approaches, including the design of culture protocols that minimize contamination risks and the identification of bacterial substrates that either promote or suppress mite proliferation. Ten bacterial species were tested: Escherichia coli, Pseudomonas spp., Enterobacter cloacae, Staphylococcus spp., Bacillusspp., Enterobacteriaceae (mixed culture), Xanthomonas euvesicatoria, Microbacterium spp., Ralstonia spp., and Pectobacteriumspp. Bacterial cultures were grown on nutrient agar plates at 30°C for 48 hours. A laboratory colony of T. putrescentiae was established from stored grain products and maintained at 25±2°C, 75±5% relative humidity, and a 12:12 photoperiod. Mite populations were monitored every two days over 17 days (March 12-28) using a completely randomized design with three replicates per treatment. Significant variation was observed in mite population growth among the different bacterial treatments. The highest mean population was recorded on Enterobacteriaceae (78.67 mites on day 9), followed by Staphylococcus (42.67) and Ralstonia(34.00). Bacillus and Xanthomonas euvesicatoria supported moderate population increases, reaching 30.00 and 24.00 mites, respectively. In contrast, Pseudomonas and Escherichia coli demonstrated poor nutritional value, with final populations of only 7.00 and 9.33 mites. These findings indicate that Enterobacteriaceae is the most suitable bacterial food source for mass rearing T. putrescentiae, offering valuable insights for improving prevention and control strategies across affected fields.