Predators impact prey populations not only through direct consumption but also a range of non-consumptive effects that influence behavior, physiology, and life-history traits. The intricate ecological interplay between predators, prey, and parasitoids is a key determinant of ecosystem dynamics. This study investigates the non- consumptive effects of the mite parasitoid Pyemotes zhonghuajia on the potato tuber moth (PTM) Phthorimaea operculella, revealing complex responses within the framework of the ‘ecology of fear’. We examined the non-consumptive effects of an ectoparasitic mite P. zhonghuajia on PTM development, growth, survival, and reproductive performances, using predatory cues from PTM larvae parasitised and killed by P. zhonghuajia mites. Gas chromatography–mass spectrometry (GC–MS) analysis was conducted to evaluate mite- emitted volatile organic compounds (VOCs). Dose-response assays, mating observations, and ovarian dissections were used to investigate dimethyl trisulfide (DMTS), a key VOC emitted by P. zhonghuajia, on PTM reproduction. PTM exhibits accelerated development and a shortened lifespan in the presence of P. zhonghuajia, particularly during the larval stage. Parasitoid exposure significantly impacts PTM’s reproductive parameters, leading toreduced fecundity. Cues from PTM larvae parasitised and killed by P. zhonghuajia mites accelerated the development of PTM eggs and larvae but reduced the egg hatching rate, larva pupation rate, and adult emergence rate. PTM larvae decreased food consumption when exposed to predation stress. Heaver body weight of females did not directly result in greater female fecundity, and predation stress had little carry-over effect on other reproductive traits of females in the parental and offspring generations. Screening of 34 volatile organic compounds revealed DMTS as the most potent oviposition suppressor; DMTS reduced egg-laying, shortened the oviposition period, and decreased female longevity, without delaying reproductive onset. DMTS suppresses mating frequency, and ovarian dissections revealed fewer mature eggs, indicating inhibition of oocyte maturation. Predatory cues and volatiles from P. zhonghuajia elicit strong non-consumptive effects in P. operculella, altering development and reducing survival and fecundity, with measurable transgenerational impacts. Among mite-emitted VOCs, dimethyl trisulfide (DMTS) is a key bioactive cue that suppresses reproduction through reduced mating, oviposition, and ovarian maturation, supporting its potential for semiochemical-based PTM management in potato storage.