The strawberry tarsonemid mite, Phytonemus pallidus (Banks), is a significant pest of strawberries in many countries, particularly in Iran. The use of a phytoseiid species that is sufficiently small to penetrate cyclamen mite habitats and naturally active at low greenhouse temperatures offers a notable advantage for effective control of strawberry mites. In this study, the efficacy of Neoseiulus longilaterus (Athias-Henriot), a predatory mite collected from the primary habitat of P. pallidus within a commercial greenhouse in Iran, was assessed for its ability to suppress P. pallidus. Its performance was compared with that of Amblyseius swirskii (Athias- Henriot) and Amblyseius herbicolus (Chant) under controlled laboratory conditions (25 ± 1 C; 80 ± 5% relative humidity; 16:8 photoperiod). Each experimental unit consisted of a single petiole leaf, with the petiole tip placed in a 10 mL glass vial filled with water. Predatory mites were released at a predator-to-prey ratio of 1:10, and assessments were conducted after 7 and 14 days. Control leaves received no predator releases. The release of all three phytoseiid mite species resulted in significant reductions in P. pallidus populations per leaf compared to the control. The most substantial suppression (91.33%) was observed following N. longilaterus release 14 days after application. Specifically, an initial population of 30 P. pallidus decreased to 3.1 and 2.6 individuals per leaf at 7 and 14 days, respectively, following N. longilaterus release. Both A. swirskii and A. herbicolus reduced P. pallidus populations by approximately 26% and 13.3% respectively, after 14 days, with no significant difference between them. The high efficacy of N. longilaterus in reducing strawberry mite populations under laboratory conditions, combined with its compatibility with the strawberry mite’s primary habitat, suggests its potential as a promising biological control agent against P. pallidus.