Nine new species of Oplitis were described and O. moseri Hirschmann redescribed. Both males and females of Oplitis exsectoidesorum, O. litoralis, O. macclellani, O. trachymyrmecon and O. virgilinus were described. Oplitis garibaldii, O. granulatus, O. piedmontensis and O. sarcinulits were described from females. A key to the species was prepared. Species were collected from colonies of the following ant genera: Brachymyrmex, Crematogaster, Formica, Lasiits, Solenopsis, Tetramoriiem and Trachymyrmex, as well as from tree hole organic matter, leaf litter and under bark.