Mites of the subfamily Nothopodinae from the Afrotropical region are dealt with. The following five new species collected on indigenous plants in South Africa are described and illustrated: Adenocolus psydraxi from Psydrax livida (Rubiaceae); Floracarus transvaalensis from Rhoicissus tomentosa (Vitaceae) and Rhus chiridensis (Anacardiaceae); F. tiliaceus from Grewia occidentalis (Tiliaceae); F. oxyanthi from Oxyanthus speciosus (Acanthaceae); Nothopoda natalensis from Aspilia natalensis (Asteraceae). The genus Adenoculus is new to science. The relationships of each species are briefly discussed.