Two new tarsonemid species, Daidalotarsonemus esalqi sp. n. and Daidalotarsonemus savanicus sp. n., found on both native and crop plants in Brazil are described herein, based on adult females. Biological aspects of these species are briefly discussed. Individuals of Daidalotarsonemus savanicus sp. n. have been misidentified as Daidalotarsonemus tessellatus De Leon in previous reports of this species from Brazil. A key is provided to distinguish females of the Daidalotarsonemus species known to occur in Brazil.