A new family of mesostigmatid mites, Euphysalozerconidae, including Euphysalozercon berlesei gen. et sp. nov. is described from the specimens deposited in the Berlese Acaroteca in Florence, Italy. This new family group name replaces the currently known monotypic Physalozerconidae, carrying Physalozercon raffrayi, which was based on an undescribed (unavailable) species name so called Antennophorus raffrayi, a provisional name proposed by Erich Wasmann. A diagnosis for the family Euphysalozerconidae and an updated key to families of Aenictequoidea are provided.