The morphological differentiations between larvae of Hyalomma anatolicum excavatum, H. dromedarii and H. marginatum marginatum were studied by Scanning Electron Microscopy (SEM) and morphometric analysis. Results showed that H. m. marginatum is easy differentiated from other two tick species while H. a. excavatum is close to H. dromedarii. Scutum is tetragonal shape in H. a. excavatum and H. dromedarii, hexagonal shape in H. m. marginatum, scutal posterior margin sinuous in H. a. excavatum and widely rounded in H. dromedarii. Hypostome is club shape in H. a. excavatum and H. dromedarii, cylindrical shape in H. m. marginatum, the dental formula is 2/2 for each tick species, the teeth numbers per file are 7/6 for each H. a. excavatum and H. dromedarii and 9/8 for H. m. marginatum. Presence of well developed spur on each coxa II and III for H. m. marginatum while this spur is very small or reduced in others. The measurements of body, Scutum, palpi, basis capituli and hypostome of H. m. marginatum larvae were significantly higher than others. H. dromedarii was significantly higher than H. a. excavtum in case of body length, scutal width, length of basis capitulum and hypostomal width.