A new species from the temple gardens on Shikoku Island, Nippon, belonging to the genus Ghilarovus of the family Zetomotrichidae (Acari) is described. The new species, Ghilarovus sanukiensis sp. nov. differs from any other congeners by its smaller body size (292 to 335 micrometers in body length); fewer rostral dents (12 to 15); the presence of lateral ridges on the prodorsum;the presence of barbs in all epimeral setae; the cilia of sensillus variable in number (15 to 16 long cilia, and 9 to 11 short ones), notogaster not separated at the posterior border; dorsal setae la as long as the lyrifissure im; the baciliform solenidion wl and famulus; and setal formular on legs.