A NEW SPECIES OF LARVAL CHARLETONIA (PARASITENGONA: ERYTHRAEIDAE) AND NEW RECORDS OF LARVAL ERYTHRAEIDAE PARASITIZING ORTHOPTERA AND PHASMIDA FROM COSTA RICA

A total of 36 larvae were captured parasitizing 4 species of Tettigoniidae (Orthoptera, Ensifera) and two phasmids (Phasmatodea) in the "Reserva Biologica Alberto Manuel Brenes" in Costa Rica. Here, we report the second capture of two species of mites since their original descriptions Leptus nikanori Haitlinger, 2000 (Erythraeidae) from Costa Rica and Charletonia domawiti Haitlinger, 2004 (Erythraeidae) from Brazil and each only known from a single specimen. We also describe and illustrate Charletonia salazari sp. nov. captured parasitizing Scopiorinus mucronatus (Saussure and Pictet, 1898) (Orthoptera, Tettigoniidae, Pseudophyllinae). A key for the species of the genus Charletonia from the New World is provided.


INTRODUCTION
We are presenting the data of mites parasitizing orthopteroids that were collected in a 15 days field trip in "Reserva Biológica Alberto Manuel Brenes (R. B.A.M.B.)" and the surrounding areas of the preserve. The preserve is located in Sierra de Tilarán, Cantón of San Ramón, Alajuela Province in Costa Rica. The specimens were captured under the auspices of the grant "Inventory of the arthropods of the Reserva Biológica Alberto Ml. Brenes" (INVAR-TRAB) and developed by the collaboration between the University of Costa Rica and I.N.I.A. (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria) (Spain).
Several orders of arthropods were collected by different specialists. Approximately, five hundred orthopteroids were sampled, either manually from vegetation or using light traps. From over these orthopteroids sampled only six were parasitized. A total of 36 larvae were removed from 4 species of Tettigoniidae (Orthoptera, Ensifera) and two phasmids (Phasmatodea) ( All the parasites captured belong to Erythraeidae Robineau-Desvoidy, 1828 and they are grouped into two genera, Leptus Latreille, 1796 and Charletonia Oudemans, 1910. These two genera are distributed world-wide but what is known for Central America is very scarce. Here, we report the second capture of two species since their original descriptions Leptus nikanori Haitlinger, 2000 from Costa Rica and Charletonia domawiti Haitliger 2004 from Brazil and both known from a single specimen; also, we describe a new species belonging to Charletonia and parasitizing Scopiorinus mucronatus.

MATERIALS AND METHODS
Mites were captured during a sampling field trip in Costa Rica while they were parasitizing different species of orthopterans. The complete list of Orthoptera identified has been published in two papers (Barranco, 2010a; Barranco, 2010b). Katydids were collected and identified, and the parasites were carefully removed in the laboratory un-der a stereomicroscope. Measurements and illustrations were made after clearing the specimens in 50% lactic acid and mounting them in PVA medium (Krantz and Walter, 2009) using a Nikon Optiphot-2 compound microscope with a drawing attachment. All the measurements are given in micrometers. Terminology and abbreviations for the description of the new species follow Saboori et al., (2009) andSedghi et al., (2010   Remarks -We found L. nikanori in two ar-eas sampled, inside the R. B.A.M.B. and in the surrounding area of Colonia Palmareña. This species was relatively common inside of the preserve. These two areas differ in the type of vegetation and in the species of orthopterans present (Barranco, 2010a, 2010b). Leptus nikanori was found on the two different hosts Phasmida and Orthoptera. We captured a total of 16 larvae parasitizing 3 species of katydids and 11 larvae parasitizing 2 species of phasmids. This species was originally described from Irazú, Costa Rica on plants (Haitlinger, 2000) from a unique larva; since morphometric data is 222 only known for the holotype, we are presenting the metric information for the 27 exemplars we captured from San Ramón, Alajuela, Costa Rica (Table 1). This is the second time that this species is captured and it is only known from Costa Rica. Remarks -These exemplars captured from Costa Rica (247A-C) are identical to the specimen known of C. domawiti (Haitlinger, 2004). This species was described from a single specimen from plants and no further captures have been reported.
Here, we provide metric data for the three specimens captured in Costa Rica ( Table 2). The only dif-ferences found between the specimens from Costa Rica and Brazil are a slightly shorter BFe I (148-158 vs. 172) and BFe III (153-166 vs. 180). This is the second report of C. domawiti, the first out of Brazil. The host is also wide-spread from south of North America to Peru and Brazil (Barranco, 2010a).
Remarks -Charletonia salazari sp. nov. belongs to the group of species with four setae between coxae II-III. This group includes species described from Europe, Africa, Asia, Australia and America. The genus Charletonia is barely known from the New World and there are 7 species reported, one from Central America, two from South America and 4 from North America (Southcott, 1991;Haitlinger, 2000;Haitlinger, 2004;Treat and Flechtmann, 1979).
All of them belong to the group with four setae between coxae II-III. The new species belongs to the species group with four setae between coxae II-III, two hypostomalae, solenidion placed distally on Ge I, fn Ge 12,12,12 and Ti III 200 -255 µm.