1Laboratory of Insect Biosystematics, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea & Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
2✉ Laboratory of Insect Biosystematics, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea & Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
3Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, Semakova Str.10, 625003 Tyumen, Russia.
2023 - Volume: 63 Issue: 3 pages: 676-690
https://doi.org/10.24349/m7r8-d4fpWithin the Laelapidae, one of the largest genera of the subfamily Hypoaspidinae, Gaeolaelaps, is well known as a group of predatory mites and the most commonly encountered laelapids in soils. Based on the few species studied, members of Gaeolaelaps prey on nematodes, acarid mites and other soft-bodied arthropods that abound in soil and decomposing organic matter. They may therefore play an important role in the balance of the soil ecosystem (Walter and Oliver 1989; Gerson et al. 2003; Lindquist et al. 2009). However, some members of the genus have occasionally been found in the nests of vertebrates (Tenorio 1982) or in association with arthropods (or their nests), including mygalomorph spiders, millipedes, ants, cockroaches, termites, as well as cerambycid, passalid, scarabaeid, carabid and heterocerid beetles (Rosario 1981; Strong and Halliday 1994; Fain et al. 1995; Mašán 1998; Trach 2012, 2016; Joharchi and Babaeian 2014; Joharchi et al. 2019a, 2019b, 2020, 2021; Saeidi et al. 2019; Moraes et al. 2022). A few species of this genus have been collected in association with passalids (Coleoptera: Passalidae) (Hyatt 1964; Hunter and Yeh 1969; Rosario 1981). Several authors have already recorded a collection of Laelapidae associated with insects and soil from the Republic of Korea (Kontschán et al. 2015, 2016; Keum et al. 2016, 2017; Joharchi et al. 2018, 2019c; Ji et al. 2023). The genus Gaeolaelaps currently comprises 160 nominal species (Moraes et al. 2022), from which only eight species have been recorded in the Korean peninsula (Kontschán et al. 2015; Joharchi et al. 2019c; Cho et al. 2021; Ji et al. 2023). However, we believe that some of these records are based on misidentifications. The present paper is part of a project that aims to increase the knowledge of the mite fauna of the Republic of Korea, particularly the poorly studied fauna of insect-associated species of mesostigmatic mites. Towards this aim, we herein describe a new species belonging to the genus Gaeolaelaps on the basis of female, male and protonymph specimens collected on an endemic bess beetle, Leptaulax koreanus (Coleoptera: Passalidae) from the Republic of Korea. In addition, an identification key to all known Korean species of Gaeolaelaps is presented, and ambiguities on the identification of some previously recorded species of the genus are discussed. Finally, the nature of the new Gaeolaelaps species-passalid associations is briefly discussed.
Host beetles, Leptaulax koreanus, were collected from various forest habitats (mostly rotten stump) by individual hand-picking (Figure 1). Mites were removed from the beetles using a fine brush, cleared in lactic acid solution and mounted in PVA medium (Downs 1943). Some beetles with mites were transferred to a rearing unit [plastic cage (23 X 14 X 18 cm in size)] with a sufficient amount of fermented sawdust and kept at in room-temperature. The line drawings and examinations of the specimens were performed with Olympus BX53 compound microscope equipped with differential interference contrast optical systems, attached to camera Olympus DP27. Most images were captured in stacks (with focal depth manually controlled). Digital drawings were prepared using Adobe Illustrator CC 22.0.1 software based on the original pencil line drawings. Images and morphological measurements were taken via cellSens Standard software (version 3.1). Photomicrographs were taken with an Olympus DP27 camera. Measurements of structures are expressed as ranges (minimum–maximum) in micrometres (μm). The length and width of the dorsal shield were taken from the anterior to the posterior margins along the midline, and at level of r3, respectively. Length and width of the sternal shield were measured at the maximum length and broadest points (at level of endopodal between coxae II and III), respectively. The length of the genital shield was measured along the midline from the anterior margin of the hyaline extension to the posterior margin of the shield, and its width was measured where broadest, posteriorly to st5. Leg length was measured from the base of coxa to the apex of tarsus, excluding the pre-tarsus. The nomenclature for the dorsal idiosomal chaetotaxy follows that of Lindquist and Evans (1965), the notations for leg and palp setae follow those of Evans (1963a, b), and other anatomical structures mostly follow Evans and Till (1979). Notations for idiosomal pore-like structures (gland pores and poroids/lyrifissures) and peritrematal shield follow mostly Athias-Henriot (1971, 1975). The notations for pore-like structures on the sternal shield and for the peritrematal shield region also follow modifications and additions by Johnston and Moraza (1991) and Moraes et al. (2022). The holotype and paratypes are deposited at the National Institute of Biological Resources (NIBR). Paratypes are also deposited in the Laboratory of Insect Biosystematics, Seoul National University (SNU), Republic of Korea.
Hypoaspis (Gaeolaelaps) Evans and Till 1966: 159.
Type species Laelaps aculeifer Canestrini, 1884, by original designation (Evans and Till 1966).
The concept of Gaeolaelaps used here is based on that of Moraes et al. (2022). More information about the synonyms and nomenclatural history of the genus are available in Moraes et al. (2022: 241).
ZOOBANK: 38DF0A34-B063-4E7E-A91D-3EF775EC6A80
(Figures 2–6)
Holotype: female, Jikdong-ri, Sohol-eup, Pocheon-si, Gyeonggi-do, Republic of Korea, 25 May 2019, J.S. Oh coll., on Leptaulax koreanus Nomura, Kon, Johki and Lee (Coleoptera: Passalidae).
Paratypes (total 33 females, two males, one protonymph): 4 females, same data as holotype; 12 females, Mt. Buyongsan, San 100-14, Minrak-dong, Euijungbu-si, Gyeonggi-do, Korea, Feb–May, 2021, taken from same host specimen of L. koreanus; 17 females, two males, and one protonymph, Laboratory condition, Seoul National University, Seoul, Korea, 2020–2021, females on L. koreanus, males and protonymph from the fermented sawdust in the cage (see material and methods).
Dorsal shield oval, length/width approx. 1.5, with weak reticulation, more distinct in the lateral and opisthonotal sections, bearing 38 pairs of setae, including two pairs of Zx setae but lacking z3; most dorsal setae relatively short, but some setae longer, especially in anterolateral areas (e.g. j3, z4, s4). Presternal platelets weakly sclerotized. Sternal shield reticulate throughout, although faintly so posteromedially. Genital shield relatively large, somewhat flask-shaped, bulging posterolaterally. Opisthogastric and lateral soft cuticle with 17 pairs of setae, including seven pairs of r-R-UR setae. Peritremes relatively long, extending to mid-level of coxae I. Epistome triangular, with pointed apex and irregularly denticulate. Fixed digit of chelicera with 12–15 teeth. Tarsus IV without noticeably macrosetae. In male, holoventral shield bearing five pairs of sternal setae and four pairs of ventral setae. Spermatodactyl straight (60–69), progressively tapering from midway into a blunt apex.
(Figures 2, 3, 5A and 5C)
Six specimens measured.
Dorsal idiosoma — (Figure 2A) — Dorsal shield oval-shaped, 950–1066 long, 632–713 wide, covering entire idiosoma dorsally; shield without distinct reticulation (faintly distinct in distinct in the lateral and opisthonotal sections, posterior to j6), bearing 38 pairs of smooth setae, including 21 pairs of podonotal setae (z3 absent) and 17 pairs of opisthonotal setae, including two pairs of Zx setae; unpaired setae Jx absent; dorsal setae mostly homogeneous in length and thickness, medial setae (j3–j6 and J1–J5) short (40–55), barely reaching more than half the distance of following setae, anteromedial setae (j1–j3) longer (60–86) than other medial setae (almost reaching bases of following setae), length of podonotal setae increasing from central to lateral (70–95); z1 (22–30) shortest, and j2, j3, z4, s3, s4 (75–95) longest, see Figure 2A. Shield with 23 pairs of discernible pore-like structures, including 16 poroids and seven gland openings (gd1, gd2, gd4–6, gd8–9).
Ventral idiosoma — (Figures 2B and 5A) — Tritosternum with paired pilose laciniae (144–155), fused basally (12–15), columnar base 54–67 long × 21–25 wide; presternal area with a pair of platelets, ornamented with two or three transverse lines, sternal shield length 184–193, narrowest between coxae II (165–178), widest at level of endopodal between coxae II and III (296–325), with reticulate ornamentation throughout, except central and posterior parts faintly reticulated (Figures 2B and 5A), anterior and posterior margins of sternal shield slightly concave, remnants of gland pores gv1 not discerned; bearing three pairs of smooth setae (st1 86–93, st2 80–86, st3 66–76), almost reaching base of next setae, and two pairs of poroids (iv1 and iv2 slit-like, mesal to setae st1 and between st2 and st3, respectively). Metasternal setae st4 (58–67) and metasternal poroids located on soft integument. Endopodal plates between coxae I–II and II–III completely fused to sternal shield, endopodal plates III/IV elongate, narrow and curved. Genital shield flask-shaped, slightly expanded laterally past level of setae st5 (Figures 2B and 5A), length 338–372, maximum width 205–245, posterior margin rounded, anterior margin of shield convex, overlapping posterior area of sternal shield, but not reaching base of setae st3, with some irregular longitudinal and oblique lines, otherwise relatively smooth, bearing a pair of simple setae st5 (55–61) inserted on lateral margins of shield, near level of posterior edge of coxae IV; paragenital poroids iv5 located on soft cuticle lateral to shield near seta st5. Anal shield pear-shaped, rounded anteriorly, length 116–138, width 114–120, anterior half lineate-reticulate, para-anal setae and post-anal seta subequal in length (38–48), cribrum consisting of a terminal tuft with three irregular rows of spicules and a pair of anterior arms extending slightly beyond post-anal seta (Figures 2B and 5A); anal gland pores (gv3) located on soft opisthogastric cuticle close to shield (Figures 2B and 5A). Soft opisthogastric cuticle with one pair of elongated metapodal plates (42–53 long × 3–6 wide) and 17 pairs of smooth setae (Jv1–Jv5, Zv1–Zv5, R1–R5, UR2–UR3) (40–60). Two subtriangular exopodal platelets between coxae II–III and III–IV present; parapodal platelets strip-like, gland pore gv2 not discerned (Figures 2B and 5A). Peritrematal shield thinly developed, bearing one gland pores (gd3) and one poroid (id3) at level near coxae II–III, poststigmatic extension of shield with two pairs of poroids (id7 and ip) and one pair of gland pores (gp) (Figures 2B and 5A); peritrematal shield fused anteriorly to dorsal shield near setae z1 (Figures 2B and 5A). Peritremes relatively long, extending to mid-level of coxae I (Figures 2B and 5A).
Gnathosoma — (Figures 2C–E and 5C) — Epistome triangular, with pointed apex and irregularly denticulate (Figure 2D). Hypostomal groove with six transverse rows of denticles, each row with 19–25 tiny denticles, with smooth posterior transverse line (Figure 2C). Hypostome with four pairs of smooth setae, h3 (125–127) < h1 (67–70) < pc (66–68) < h2 (64–68) (Figure 2C). Corniculi robust and horn-like, extending slightly beyond palptrochanter. Internal malae with one pair of smooth median projections, flanked by lobes with fimbriate anterior margin; labrum with pilose surface (Figure 2C); supralabral process not distinct. Chaetotaxy of palps: trochanter 2, femur 5, genu 6, tibia 14, tarsus 15, all setae smooth; palpfemur with seta d3 thickened and al paddle-like; palpgenu with al1 stout, blunt, al2 thickened and spatulate; palptarsal apotele two-tined. Fixed digit of chelicera with an offset distal tooth (gabelzhan), followed by 12–15 various sized teeth (mostly small), a setaceous pilus dentilis, dorsal cheliceral seta small and prostrate, arthrodial membrane with a rounded flap and normal filaments; cheliceral dorsal and lateral lyrifissures distinct; movable digit with two mid-sized teeth (Figures 2E and 5C).
Insemination structures – Laelapid-type sperm access system; tubulus long, wider at near the solenostome, at level of coxa III. Proximal ends of the tubulus swollen at junction with ramus, leading to irregular sacculus.
Legs — (Figure 3) — Legs II and III short (771–808, 797–860), I and IV longer (980–1060, 1090–1130). Chaetotaxy normal for free-living Laelapidae: Leg I (Figure 3A): coxa 0-0/1, 0/1-0, trochanter 1-1/1, 0/2-1 (ad slightly thickened), femur 2-3/1, 2/3-2, genu 2-3/2, 3/1-2, tibia 2-3/2, 3/1-2. Leg II (Figure 3B): coxa 0-0/1, 0/1-0, trochanter 1-0/2, 0/1-1, femur 2-3/1, 2/2-1 (pd2 thickened and slightly longer than others), genu 2-3/1, 2/1-2 (pv slightly thickened), tibia 2-2/1, 2/1-2 (pv slightly thickened). Leg III (Figure 3C): coxa 0-0/1, 0/1-0, trochanter 1-1/1, 0/1-1, femur 1-2/1, 1/0-1 (pd slightly thickened), genu 2-2/1, 2/1-1 (all ventral setae slightly thickened), tibia: 2-1/1, 2/1-1 (all ventral setae slightly thickened). Leg IV (Figure 3D): coxa 0-0/1, 0/0-0, trochanter 1-1/2, 0/1-0, femur 1-2/1, 1/0-1 (ad1 longest and inserted on small tubercles), genu 2-2/1, 3/0-1 (av slightly thickened), tibia 2-1/1, 3/1-2 (all ventral setae thickened). Tarsi II–IV with 18 setae (3-3/2, 3/2-3 + mv, md); with some ventral and lateral setae thickened, tarsus of leg IV without noticeably longer setae (macrosetae), see Figure 3. All pretarsi with well-developed paired claws, rounded pulvilli and normal ambulacral stalk.
(Figures 4, 5B and 5D)
Two specimens measured.
Dorsal idiosoma — Dorsal shield 731–751 long, 440–506 wide; ornamentation and chaetotaxy as in female.
Ventral idiosoma — (Figures 4A and 5B) — Sternal, genital, endopodal, ventral and anal shields fused into holoventral shield, 515–520 long from anterior to posterior margins of shield, 140–147 wide at level of st2, 156–162 at st3 level and 280–285 at broadest point, posteriorly to coxae IV; shield reticulate throughout (ornamentation only partly drawn on Fig. 4A), with five pairs of simple sternal setae (st1–5), and four pairs of smooth ventral setae (Jv1, Jv2, Zv1, Zv2), plus three smooth circumanal setae, para-anal setae and post-anal seta subequal in length, with five pairs of poroids; gland pore gv2 behind coxa IV not discerned; cribrum with 3–4 irregular rows of spicules; metapodal platelets narrow and fused to holoventral shield (Figures 4A and 5B). Soft opisthogastric and lateral cuticle with 10–12 pairs of setae. Peritremes, peritrematal shields and other ventral structures similar to those in female.
Gnathosoma – (Figures 4B and 5D) — Fixed digit of chelicera with two teeth of various sizes in addition to apical hook, pilus dentilis setaceous, slightly thicker than in female. Movable digit of chelicera with a relatively large tooth, spermatodactyl straight, progressively tapering from midway into a blunt apex, free portion of spermatodactyl almost as long as movable digit (90% of movable digit length at most) (60–69).Other gnathosomal structures similar to those in female.
Legs – Chaetotaxy as in female.
(Figure 6)
One specimen measured.
Dorsal idiosoma — (Figure 6A) — Whitish, oval (646 long × 463 wide), with podonotal (414 long × 368 wide) and pygidial (171 long × 240 wide) shields, three pairs of mesonotal scutellae, the largest 54 long × 20 wide, and six pairs of setae located between podonotal and pygidial shields (J1, J2, Z1, Z2, S2, S3) (Figure 6A). Podonotal shield weakly reticulated, only distinct in lateral regions, with 11 pairs of simple setae (j1–6, z2, z4, z5, s4–5) (r2, r5 and R1 on lateral soft cuticle), medial setae (j4–6, z5) shorter (30–40), other setae longer (55–65). Pygidial shield faintly reticulated throughout, with eight pairs of simple setae (J3–5, Z3–5, S4–5). All setae similar in length (30–40) except S4, S5 and Z5 longer (45–55) (Figure 6A).
Ventral idiosoma — (Figure 6B) — Sternal shield distinct, length 270, narrowest between st1 and st2 (110), widest between st2 and st3 (160), with three pairs of smooth setae (st1–3, 50–55) and sternal poroids, metasternal setae st4 absent. Anal shield length 85, width 75, cribrum consisting of irregular rows of spicules extending slightly beyond post-anal seta. Opisthogastric cuticle with five pairs of smooth setae (st5, Jv1–2, Jv5, Zv2). Peritreme short, located in the region of coxae III/IV (Figure 6B), peritrematal shield and post-stigmatic section not developed.
Gnathosoma — similar to the adult.
Legs — (Figures 6C–F) — Legs II (526) and III (536) short, I (621) and IV (700) longer. Chaetotaxy: Leg I (Figure 6C): coxa (2) 0-0/1, 0/1-0, trochanter (4) 1-0/2, 0/1-0, femur (10) 2-2/1, 2/1-2, genu (8) 1-2/1, 2/1-1, tibia (8) 1-2/1, 2/1-1. Leg II (Figure 6D): coxa (2) 0-0/1, 0/1-0, trochanter (4) 1-0/1, 0/1-1, femur (8) 1-2/1, 2/1-1, genu (6) 1-2/0, 2/0-1, tibia (7) 1-1/1, 2/1-1. Leg III (Figure 6E): coxa (2) 0-0/1, 0/1-0, trochanter (4) 1-1/1, 0/1-0, femur (5) 1-2/1, 1/0-0, genu (6) 1-2/0, 2/0-1, tibia (7) 1-1/1, 2/1-1. Leg IV (Figure 6F): coxa (1) 0-0/1, 0/0-0, trochanter (4) 1-1/1, 0/1-0, femur (4) 1-2/0, 1/0-0, genu (5) 1-2/0, 2/0-0, tibia (7) 1-1/1, 2/1-1. Tarsi II–IV with 17 setae (3-3/2, 3/2-3 + md); most setae on ventral and lateral aspects thickened (or spine-like). All pretarsi with well-developed paired claws, rounded pulvilli and normal ambulacral stalk.
The specific name of this species is treated as a noun in apposition and refers to its occurrence on beetles of the genus Leptaulax.
Since all specimens of new species were found on passalids, this strongly suggests that there is a biological link between the mite species and the passalid host. Thus, the possibility of accidental phoresy can be ruled out. Prior to our work, only five species regarded to belong to Gaeolaelaps have been reported on bess beetles (Coleoptera: Passalidae) (Moraes et al. 2022): Gaeolaelaps circularis Hyatt, 1964; G. disjuncta (Hunter and Yeh, 1969); G. paraculeifer Rosario, 1981; G. passalus Rosario, 1981; and G. rarosae Rosario, 1981. Gaeolaelaps leptaulax can be easily distinguished from all of these species by its rather large body size (ca. 950–1066 in length, 632–713 in width), dorsal shield with 38 pairs of setae (z3 absent), and presternal area with a pair of platelets, while all five above-mentioned species have smaller body size, with 37 pairs or fewer dorsal setation and presternal area without platelets (= with a few transverse curved lines) (except for G. disjuncta there is a similar pair of platelets in sternal region).
Kontschán et al. (2015) reported G. kargi (Costa, 1968) from the Democratic People's of Republic of Korea basis on one specimen collected from litter. We have had the opportunity to examine that specimen deposited in NIBR, but the species identity of the specimen could not be confirmed due to its poor condition. Therefore, the record of G. kargi remains unconfirmed. Gaeolaelaps praesternalis (Willmann, 1949) has also been recorded by Kontschán et al. (2015) (as Hypoaspis presternalis [sic]) from moss and lichen samples collected in the Democratic People's of Republic of Korea. We have not had the opportunity to examine any specimens to confirm this identification. Nevertheless, based on the illustration of the specimen provided by Kontschán et al. (2015: 36, Figure 1e), as well as comparing it with supplementary descriptions and photomicrographs of G. nolli and G. praesternalis which were based on the type series of the species (see Joharchi and Negm, 2020: 499), we believe that this was a misidentified specimen of Gaeolaelaps nolli (Karg, 1962). There is some confusion about the identification of these two species, and that was discussed by Joharchi and Negm (2020: 499), who listed the main differences of these two species by making a list of differences between G. nolli and G. praesternalis. The following key is based on the direct examination of specimens of all species included.
1. Peritreme long, reaching coxa I
...... 2
— Peritreme short, reaching coxa II
...... 7
2. Palp tarsal claw three-tined; dorsal shield caudally with a curvature (tapering caudally)
...... G. queenslandicus (Womersley, 1956)
— Palp tarsal claw two-tined; dorsal shield not tapering caudally and without any curvature in posterior part
...... 3
3. Dorsal shield with 39 pairs of setae, including z3
...... 4
— Dorsal shield with 38 pairs of setae, lacking z3
...... 6
4. Sternal seta st1 inserted in presternal region
...... 5
— Sternal seta st1 inserted on sternal shield
...... G. aculeifer Canestrini, 1884
5. Dorsal shield obviously narrowing from level of setae r3; progressively tapering, genital shield not abuts the anal shield, the distance between the genital and anal shields almost as long as anal shield
...... G. aculeiferoides (Teng, 1982)
— Dorsal shield oval-shaped; genital shield closely abuts the anal shield
...... G. debilis (Ma, 1996)
6. Tarsus IV with two elongate setae, idiosoma mid-sized, dorsal shield parallel-sided, all dorsal setae mid-sized and subequal; opisthogastric and lateral soft cuticle with only eight pairs of setae
...... G. kargi Costa, 1968
— Tarsus IV without elongate setae, idiosoma large, dorsal shield oval-shaped, with rounded lateral margins, some anterolateral dorsal setae are longer in contrast to short posteromedian setae; opisthogastric and lateral soft cuticle with 17 pairs of setae
...... G. leptaulax n. sp.
7. Dorsal shield with 39 pairs of setae, including two pairs of Zx setae
...... 8
— Dorsal shield with 37 pairs of setae, lacking Zx setae
...... G. zhoumanshuae (Ma, 1997)
8. Fixed digit of female chelicera with four teeth, tarsus IV without elongated macrosetae
...... G. similisetae (Karg, 1965)
— Fixed digit of female chelicera with six teeth, tarsus IV with two elongated macrosetae (pd2–3)
...... G. nolli (Karg, 1962)
Passalids live in hardwood logs and exhibit subsocial behavior quite unusual for beetles. Both bess beetle parents live in their log homes with their offspring, and family members guard their home against intruders. The beetles dig tunnel systems in rotting wood where the females then lay their eggs and care for their young by preparing food for them and helping the larvae build the pupal case (Hadley 2021). The ecological role of G. leptaulax is unknown. Nevertheless, the specimens of G. leptaulax collected have a morphology largely typical of free-living Gaeolaelaps (e.g. dentate chelicerae; sclerotized, horn-like corniculi, well-developed deutosternum, almost holotrichous dorsal chaetotaxy), and no specimens were considerably swollen, overall suggesting that this species is not parasitic. For that reason, we suspect that G. leptaulax has a commensal or mutualistic relationship with its beetle host, perhaps feeding on other small invertebrates such as astigmatic mites or nematodes in its rotting wood nest. If they prey on parasitic invertebartes, then the relationship could be mutualistic. Alternatively, commensal or even antagonistic relationships are possible if their preferred bess beetles eggs or larvae, and they feed either on substances around the egg masses or larvae of their beetle host (as harmless feeders) or on the eggs and larvae themselves. Regardless of their habits, we stress the true ecological role of these mites must be determined experimentally.
We appreciate Prof. Kyeong-yeoll Lee, Lab. of Insect Molecular physiology, Kyungpook National University (Daegu, Korea), and also Prof. Chuleui Jung and Dr. Seoyoung Keum, Lab. of Insect Ecology, Andong National University (Andong, Korea), for the provided mite specimens to examination. Additionally, we are grateful to the National Institute of Biological Resources for the loan of the specimen. This study was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202102203). The study of Omid Joharchi was supported by the cooperative agreement No. FEWZ-2021–0004 from the Russian Ministry of Science and Higher Education. We cordially thank Fred Beaulieu and Viacheslav A. Trach for their valuable comments on this paper.