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Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae)

Noel, V.; Leger, E.; Gomez-Diaz, E.; Risterucci, A.-M. and McCoy, K.D.

2012 - Volume: 52 Issue: 2 pages: 123-128

DOI: 10.1051/acarologia/20122041
Date received: 2012-01-02, Date accepted: 2012-03-01, Date published: 2012-06-22

(CC BY-NC-ND 2.0) © 2012 Noel, V.; Leger, E.; Gomez-Diaz, E.; Risterucci, A.-M. and McCoy, K.D.

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Ectoparasite; Genetic markers; Population genetics; Tick-borne disease
Nine microsatellite markers were isolated from unfed larvae of Ixodes ricinus and were tested on two populations of nymphs collected on roe deer (N=21) and birds (N=39) in a French suburban forest. All markers were polymorphic, with limited evidence for deviations from linkage equilibrium. In accordance with previous markers developed for this species, we found large heterozygote deficits for six of the nine loci. Deficits were of the same order of magnitude within a tick infrapopulation, suggesting that population-level estimates were not due to a Wahlund effect among individual hosts, but more likely to technical problems (i.e., null alleles due to mutations in the flanking regions of the microsatellites). Although micro-geographic substructure (e.g., homogamy within infrapopulations) can not be ruled out, it is possible that null alleles could be an inherent problem associated with this tick species and specific genome-level studies are called for. Despite the possible presence of null alleles, the precision of population genetic estimates was improved by the addition of the newly-developed markers making them a useful addition for studying the population ecology of I. ricinus.

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Photos: Varroa destructor (Varroidae) on a honey bee. Low temperature -Scanning electron microscopy photograph. Material collected and fixed for LT-SEM: J. Pettis, USDA-ARS-Bee Lab. and R. Ochoa, USDA-ARS-SEL; LT-SEM photo: E. Erbe and C. Pooley, USDA-ARS-CEMU © USDA; Petrobia harti (Tetranychidae) © INRA - Alain Migeon; Dermanyssus gallinae (Dermanysssidae) © Ecole Nationale Vétérinaire de Lyon - Lise Roy; Adult Ixodes sp. (Ixodidae) engorging on an Atlantic puffin Fratercula arctica © Andy Darrington; Panonychus ulmi (Tetranychidae): hatching of a winter egg © Université de Lausanne - Centre de microscopie électronique - Cazelles
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