The unsaponifiable matter (USM) and the total fatty acid methyl eater (FAME) fractions of Glossostemon bruguieri (Desf.) growing in Egypt were determined far seeds, leaves and roots. Qualitative and quantitative variations were found by GLC analysis of the USM of the three botanical parts. Analysis of FAME revealed that palmitic, stearic, oleic and linoleic were the main fatty acids in the investigated botanical parts. Moreover, acaricidal activity of the unsaponifiable matter of each organ was performed, from which the leaves was the most toxic to both adult and egg stages of Tetranychus urticae Koch.