Two species of Eremaeozetidae, found on the Galapagos Islands (Ecuador), are described and illustrated. Eremaeozetes irenae sp. nov. has been found on four islands of the Galapagos archipelago in arid habitats, while E. darwini sp. nov. occurs frequently in epiphytic moss and organic litter in the higher zones of different islands. Notes on the distribution and ecological preferences of these species are included. Juvenile instars of both species are described. This is the first time that immatures of Eremaeozetes species have been described.