|SE|S|AM|E| Barcode
SEquences Seeker & AMplicons Explorer for Barcoding

Genotyping and barcoding based on
high-throughput multiplex amplicon sequencing

The |SE|S|AM|E| Barcode team:

Development: Sylvain Piry2, Emese Meglécz1, Audrey Realini2

Documentation and quality insurance: Emmanuel Guivier2

Consultants: Erick Desmarais3, Maxime Galan2, André Gilles1, Nicolas Pech1

Supervisor: Jean-François Martin2

1Aix-Marseille Université, CNRS, IRD, UMR 6116 - IMEP, Equipe Evolution, Génome et Environnement, 13331 Marseille Cedex 3, France, 2UMR CBGP (INRA / IRD / Cirad / Montpellier SupAgro), Campus international de Baillarguet, CS 30016, F-34988 Montferrier-sur-Lez cedex, France, 3Institut des Sciences de l'Evolution - Montpellier (I.S.E.-M.), Unité Mixte de Recherche UMII - CNRS (UMR 5554), Université de Montpellier II - CC 065, 34095 MONTPELLIER Cedex 05, France.

Disclaimer and licence: The software on this page is free to download and use, and thus comes with no warranty of any kind. While it hasn't caused us any problem, current version of |SE|S|AM|E| Barcode is still considered as beta version and you are responsible for any damages or loss of data you may sustain while using this software.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.




References to Cite:

Meglecz E, Piry S, Desmarais E, et al. (2011) SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing. Bioinformatics 27, 277-278

Piry S, Guivier E., Realini A & Martin J.-F. (2012) |SE|S|AM|E| Barcode: a NGS-oriented software for amplicon characterization - application to species and environmental barcoding. Molecular Ecology Resources, 12(6) 1151-1157

Overview:

|SE|S|AM|E| Barcode is a user-friendly web application for barcoding amplicon sequences obtained through NGS. The software automatically processes DNA sequences datasets, sorting for multiplexed loci and samples identified by oligonucleotide tags, and allows for the assignment of sequences to a preloaded reference library (from Genbank to in-house scale). The results are displayed both as samples-based synthetic tables and graphical representations. The deliveries of |SE|S|AM|E| Barcode are though with multiple applications in mind, such as specimen assignment to species referenced in DNA libraries, molecular characterization of trophic networks or more generally metagenomics and environmental barcoding studies.

It was tested extensively with Mozilla Firefox and Chrome web browsers as available the 15 february 2012.

Download |SE|S|AM|E| Barcode and documentations:

Windows
Linux
SESAME Barcode software for Windows SESAME Barcode software for Linux
Installation instructions Installation instructions
User Guide - Example dataset User Guide - Example dataset
Virtual Machine 32bits (demo)
Virtual Machine 64bits (production)
SESAME Barcode as a virtual machine 32bits SESAME Barcode as a virtual machine 64bits
Installation instructions Installation instructions
User Guide - Example dataset User Guide - Example dataset - Last available WAR file