TILLING is a reverse genetic strategy to identify mutations throughout a genome and a screening method that facilitates localization of these mutations. The mutations are induced by the chemical mutagen EMS (ethyl methane sulfonate) and particular regions can be screened for the presence of mutations by high-throughput PCR. This procedure has been successfully developed for Arabidopsis (see http://tilling.fhcrc.org:9366/home.html for experimental details and advice), and is currently being extended to a wide variety of organisms including maize, rice, pea, lotus, tomato, Drosophila and Zebrafish.
See http://tilling.fhcrc.org:9366/files/TILLING_publications.html for a list of TILLING-related publications.
The European Medicago truncatula TILLING project is funded by the GLIP (EU-Grain legumes Integrated Project). The populations have been generated by INRA- URLEG (Grain Legume Genetics and Ecophysiology Research Unit) in Dijon. The establishment of a high-throughput TILLING service is a collaborative effort between the INRA-URLEG lab in Dijon (France) and the Genome Lab in Norwich (UK).